FastQCFastQC Report
Mon 6 Jun 2016
ERR1211178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6787622
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262540.3867923110626962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222800.32824456046609546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144670.21313797379995525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147700.047.883051
GTATCAA260350.039.6813961
GTACTAG59950.036.8657571
TACCTGG37600.035.626752
GGGCACC66000.035.1157794
ATCAACG305000.032.5823173
CAACGCA305450.032.5345955
ACCTGGG41700.031.2262973
CTGTGCG80050.031.16759
TAGGCAT75450.030.3903525
TATCAAC337400.030.3690092
AACGCAG342350.029.741566
TAGTACT88500.029.1081164
ACTGTGC92400.028.8323388
TCAACGC351000.028.6069074
GTGGTAT51850.027.9329221
CTAGGCA84500.027.7600754
GCATCGT73500.027.6802068
GTACATG340850.027.3573421
TACATGG342850.026.9935972
Produced by FastQC (version 0.11.5)