FastQCFastQC Report
Mon 6 Jun 2016
ERR1211177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748083
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263880.3016432285793356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209490.23946960722709193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128690.14710651465012392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148450.046.2228161
GTATCAA260800.039.6822931
CAACGCA294100.033.5503435
ATCAACG300350.032.9459153
TATCAAC323700.031.7529492
GTACTAG67800.031.4447081
AACGCAG331800.030.3049776
GCATCGT64950.030.0342088
GTGGTAT55650.029.9376141
TCAACGC340850.029.3484654
GTACATG360950.028.0460991
ACGCAGA358250.027.5167247
TACATGG364350.027.151142
TAGGCAT83650.027.0307875
CGCAGAG365450.026.9722848
CTGTGCG87050.026.5141289
ACATGGG363000.026.508693
TAGTACT93900.025.1813764
GCAGAGT393350.024.5343449
GGGCACC79600.024.44205394
Produced by FastQC (version 0.11.5)