FastQCFastQC Report
Mon 6 Jun 2016
ERR1211177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748083
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233970.26745288081971785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198050.22639245649589745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124600.14243120464220563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145100.047.964591
GTATCAA252300.039.7371141
CAACGCA297550.032.12035
ATCAACG298100.032.0100673
TATCAAC325400.030.679782
AACGCAG335650.029.188256
TCAACGC343100.028.250134
GGGCACC82950.027.77006594
CTGTGCG93050.027.6724669
GTGGTAT51800.026.326591
ACGCAGA362700.026.2988727
TAGGCAT84500.026.1910465
TAGTACT100700.026.0002924
GTACATG351000.025.749731
CGCAGAG370950.025.650358
TAACGCC29150.025.6395914
CTAACGC29600.025.4086023
TACATGG352000.025.1566892
ACATGGG340850.024.8785883
TCTAACG30300.024.8194772
Produced by FastQC (version 0.11.5)