FastQCFastQC Report
Mon 6 Jun 2016
ERR1211176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10219727
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369980.3620253261168327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271860.2660149336670148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179880.17601252949320467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191050.047.3169671
GTATCAA347300.040.920291
GTACTAG105150.036.4741251
GCATCGT96100.035.853558
GGGCACC111200.034.7827394
CAACGCA396150.034.0796785
ATCAACG398650.033.783583
TATCAAC444300.031.785142
CTGTGCG132800.031.7496919
TAGGCAT128700.031.557745
AACGCAG458300.030.1663116
TAGTACT141950.030.101974
ACTGTGC149050.029.6131888
GTGGTAT61400.029.1240521
GTACATG505300.029.0470921
CTAGGCA145550.028.4854324
TCAACGC482650.028.3125614
TACATGG512150.028.3001022
ACATGGG499750.027.7768783
ACGCAGA486050.027.767437
Produced by FastQC (version 0.11.5)