##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211176_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10219727 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72729095405386 31.0 31.0 34.0 30.0 34.0 2 31.759847205311843 33.0 31.0 34.0 30.0 34.0 3 31.82953771661415 33.0 31.0 34.0 30.0 34.0 4 35.43397304057144 37.0 35.0 37.0 33.0 37.0 5 35.49454608719 37.0 35.0 37.0 33.0 37.0 6 35.553878004764705 37.0 35.0 37.0 33.0 37.0 7 35.47655509780252 37.0 35.0 37.0 33.0 37.0 8 35.4917264423991 37.0 35.0 37.0 33.0 37.0 9 37.095376324631765 39.0 37.0 39.0 33.0 39.0 10-11 37.056163975808744 39.0 37.0 39.0 33.0 39.0 12-13 36.95105881986867 39.0 37.0 39.0 33.0 39.0 14-15 38.174962207894595 40.0 38.0 41.0 33.0 41.0 16-17 38.16400849063776 40.0 38.0 41.0 33.0 41.0 18-19 38.18650204648324 40.0 38.0 41.0 33.0 41.0 20-21 38.15873012067739 40.0 38.0 41.0 33.0 41.0 22-23 38.04787065251352 40.0 38.0 41.0 33.0 41.0 24-25 37.94901703342956 40.0 38.0 41.0 33.0 41.0 26-27 37.80171852927187 40.0 37.0 41.0 32.5 41.0 28-29 37.69430039569551 40.0 37.0 41.0 32.0 41.0 30-31 37.54498417619179 40.0 37.0 41.0 32.0 41.0 32-33 37.3695577680304 40.0 36.0 41.0 32.0 41.0 34-35 37.22807845062789 40.0 36.0 41.0 31.0 41.0 36-37 37.120033147656486 39.5 36.0 41.0 31.0 41.0 38-39 36.969937993451296 39.0 36.0 41.0 31.0 41.0 40-41 36.80299385688092 39.0 35.0 41.0 30.0 41.0 42-43 36.586752904456254 39.0 35.0 41.0 30.0 41.0 44-45 36.338398912221436 39.0 35.0 40.0 29.5 41.0 46-47 36.07694055819691 38.5 35.0 40.0 28.5 41.0 48-49 35.83766239548278 38.0 35.0 40.0 28.0 41.0 50-51 35.132881484994655 37.5 33.5 39.5 26.5 40.5 52-53 35.219651611045975 38.0 34.0 40.0 27.5 40.5 54-55 35.67626282972138 38.0 35.0 40.0 28.0 41.0 56-57 35.60259584233512 38.0 34.5 40.0 28.0 41.0 58-59 35.36435699309776 38.0 34.0 40.0 27.5 41.0 60-61 35.14181959067987 37.0 34.0 40.0 27.0 41.0 62-63 34.835911957335064 37.0 34.0 40.0 26.5 41.0 64-65 34.585489025293924 36.0 34.0 40.0 26.0 41.0 66-67 34.41836949264888 36.0 34.0 39.0 26.0 41.0 68-69 34.1364147007058 35.0 33.0 39.0 26.0 41.0 70-71 33.871367014011234 35.0 33.0 39.0 26.0 41.0 72-73 33.56610709855557 35.0 33.0 38.0 25.5 40.0 74-75 33.24029012712375 35.0 33.0 37.0 25.0 40.0 76-77 32.933967854522926 35.0 33.0 37.0 24.5 39.0 78-79 32.614762165369 35.0 32.0 36.0 24.0 39.0 80-81 32.337807996241 35.0 32.0 36.0 24.0 38.5 82-83 32.04937896090571 35.0 32.0 35.5 23.0 37.0 84-85 31.80909499833019 35.0 31.5 35.0 23.0 37.0 86-87 31.589290105303206 35.0 31.0 35.0 22.0 36.5 88-89 31.392342280767384 35.0 31.0 35.0 20.5 36.0 90-91 31.16440571259878 34.0 31.0 35.0 20.0 36.0 92-93 30.965709504764657 34.0 31.0 35.0 20.0 36.0 94-95 30.805323322237474 34.0 31.0 35.0 19.0 35.0 96-97 30.622920944952835 34.0 30.0 35.0 19.0 35.0 98-99 30.399371235650424 34.0 30.0 35.0 18.0 35.0 100 29.250785759737028 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3761950404135561 1101 2 -0.3509323812153795 1101 3 -0.3491525701616176 1101 4 -0.23995706599334454 1101 5 -0.2242862504557479 1101 6 -0.19745279721012565 1101 7 -0.2935131368752977 1101 8 -0.3132142494370882 1101 9 -0.41505620445888525 1101 10-11 -0.4069447596036966 1101 12-13 -0.4019265452684593 1101 14-15 -0.5592187795096564 1101 16-17 -0.5740121890088616 1101 18-19 -0.5292028561105724 1101 20-21 -0.5695575483980946 1101 22-23 -0.6292174051214161 1101 24-25 -0.6561313639876261 1101 26-27 -0.6337584146927568 1101 28-29 -0.5949416895668591 1101 30-31 -0.6553557895027495 1101 32-33 -0.708630584046702 1101 34-35 -0.7208780362809151 1101 36-37 -0.7239949640362653 1101 38-39 -0.7030938654315122 1101 40-41 -0.7859211606201981 1101 42-43 -0.8604610173490101 1101 44-45 -0.8494628467919227 1101 46-47 -0.9932540947223956 1101 48-49 -0.9102469777937827 1101 50-51 -0.9446518743840429 1101 52-53 -0.920972952242785 1101 54-55 -0.7563767518802962 1101 56-57 -0.6918096701326917 1101 58-59 -0.7492825677808739 1101 60-61 -0.6336434965459858 1101 62-63 -0.7155112113677191 1101 64-65 -0.671922501586856 1101 66-67 -0.7004339755952032 1101 68-69 -0.7138035259222235 1101 70-71 -0.782327779067785 1101 72-73 -0.7563198822393105 1101 74-75 -0.8109719841639773 1101 76-77 -0.823262382030741 1101 78-79 -0.8090859437291087 1101 80-81 -0.8881473971084155 1101 82-83 -0.8981679954435897 1101 84-85 -0.9502780708312173 1101 86-87 -0.9470071851943658 1101 88-89 -1.0090516969140069 1101 90-91 -1.0335440263522742 1101 92-93 -1.158698012341464 1101 94-95 -1.2308589961070737 1101 96-97 -1.3077623084658825 1101 98-99 -1.3290802938311153 1101 100 -1.3674483099491468 1102 1 -0.4238649157877461 1102 2 -0.42617942062604186 1102 3 -0.3966084549879838 1102 4 -0.18463976143040384 1102 5 -0.25703681530218603 1102 6 -0.31512600384065337 1102 7 -0.2493718616887861 1102 8 -0.29137988748007615 1102 9 -0.4407556676030495 1102 10-11 -0.44269642777297236 1102 12-13 -0.46446502396405265 1102 14-15 -0.6302962635346816 1102 16-17 -0.6053796975417285 1102 18-19 -0.5970731285122213 1102 20-21 -0.6437502844948924 1102 22-23 -0.6049959304660959 1102 24-25 -0.6421047227589511 1102 26-27 -0.7121630325006194 1102 28-29 -0.7633883441090603 1102 30-31 -0.7337543661721782 1102 32-33 -0.7446868023055586 1102 34-35 -0.7623328843328565 1102 36-37 -0.8394531249334563 1102 38-39 -0.880268545812136 1102 40-41 -0.8704362461099819 1102 42-43 -0.8789201664997393 1102 44-45 -0.9420006600033801 1102 46-47 -0.9057109988710081 1102 48-49 -0.8841649283941493 1102 50-51 -0.9985692074458115 1102 52-53 -0.856784782479501 1102 54-55 -0.7031148727487135 1102 56-57 -0.7341073624753491 1102 58-59 -0.6985135308591595 1102 60-61 -0.6563763004454515 1102 62-63 -0.7082729686217419 1102 64-65 -0.7764988461381108 1102 66-67 -0.7848260328214636 1102 68-69 -0.8624944304033946 1102 70-71 -0.819097963997578 1102 72-73 -0.8589406431580926 1102 74-75 -0.9073115912675078 1102 76-77 -0.904965634023501 1102 78-79 -0.9495333024954391 1102 80-81 -1.0055507278661153 1102 82-83 -0.9678449901555801 1102 84-85 -0.993766020869657 1102 86-87 -1.060518354300406 1102 88-89 -1.104919097952969 1102 90-91 -1.135655912455146 1102 92-93 -1.1135073242864841 1102 94-95 -1.1190113455633437 1102 96-97 -1.1818388053880682 1102 98-99 -1.3303979716021637 1102 100 -1.3253030268987978 1103 1 -0.40872211626612653 1103 2 -0.3373614344637197 1103 3 -0.4166203102507957 1103 4 -0.3045444044147416 1103 5 -0.2993330092969515 1103 6 -0.31252121747751715 1103 7 -0.35626035785421095 1103 8 -0.35577131221417346 1103 9 -0.523416763399581 1103 10-11 -0.4769841894515423 1103 12-13 -0.5187145288198636 1103 14-15 -0.6253485302866792 1103 16-17 -0.6630913191933416 1103 18-19 -0.5773422380740953 1103 20-21 -0.6644428672319407 1103 22-23 -0.7009521150330471 1103 24-25 -0.6832945890225091 1103 26-27 -0.7058618438480764 1103 28-29 -0.8057960719750028 1103 30-31 -0.7463210673502871 1103 32-33 -0.8658600013201081 1103 34-35 -0.8552001109063028 1103 36-37 -0.8173632885225999 1103 38-39 -0.864999408665625 1103 40-41 -0.9062053945968458 1103 42-43 -0.9939805799137105 1103 44-45 -1.0385477687352633 1103 46-47 -1.0003629206311757 1103 48-49 -1.0195629103023052 1103 50-51 -1.1203742784823731 1103 52-53 -1.058840389424212 1103 54-55 -0.7607380703227378 1103 56-57 -0.749258529336025 1103 58-59 -0.8063937252756617 1103 60-61 -0.7795712079541701 1103 62-63 -0.777307101848173 1103 64-65 -0.7048435596655551 1103 66-67 -0.8347109129478412 1103 68-69 -0.8178066792682799 1103 70-71 -0.7910969856625556 1103 72-73 -0.777837190358774 1103 74-75 -0.8036432946554513 1103 76-77 -0.8605380366390136 1103 78-79 -0.8395655336080878 1103 80-81 -0.8767163564310465 1103 82-83 -0.9062216221698165 1103 84-85 -0.9073280676004813 1103 86-87 -0.9809173752297866 1103 88-89 -1.018416933785165 1103 90-91 -1.1591652234951901 1103 92-93 -1.2180960588444485 1103 94-95 -1.2076630576802927 1103 96-97 -1.2441787302230054 1103 98-99 -1.2751910347409385 1103 100 -1.2939513736903656 1104 1 -0.4358881447001046 1104 2 -0.4371601349222729 1104 3 -0.5012538291448898 1104 4 -0.30147500648184433 1104 5 -0.2691879828910899 1104 6 -0.26668776512316583 1104 7 -0.32899485585718224 1104 8 -0.2762035517303829 1104 9 -0.4144992609253464 1104 10-11 -0.4180140936914398 1104 12-13 -0.38829964676829576 1104 14-15 -0.6172731084902026 1104 16-17 -0.7234363177062733 1104 18-19 -0.7183209772802854 1104 20-21 -0.6660761155982726 1104 22-23 -0.6039038921988222 1104 24-25 -0.6954498551562978 1104 26-27 -0.6685823617827253 1104 28-29 -0.7417702155697938 1104 30-31 -0.7394289104710623 1104 32-33 -0.7440691579287986 1104 34-35 -0.9355042614071181 1104 36-37 -0.8859259459453881 1104 38-39 -0.8623426915956571 1104 40-41 -0.8954882103707931 1104 42-43 -0.7963135038147584 1104 44-45 -0.9626603620547414 1104 46-47 -1.034176370733924 1104 48-49 -0.9051826793287248 1104 50-51 -1.0213570840065174 1104 52-53 -0.8678855104307743 1104 54-55 -0.7628740634536797 1104 56-57 -0.7815817243193308 1104 58-59 -0.7645861797354243 1104 60-61 -0.756063605451871 1104 62-63 -0.7989378129665212 1104 64-65 -0.7411995506793261 1104 66-67 -0.8467456177953281 1104 68-69 -0.881317692772825 1104 70-71 -0.8168057768067172 1104 72-73 -0.7028890695185623 1104 74-75 -0.7529082488757766 1104 76-77 -0.7533707311795581 1104 78-79 -0.8136602317679937 1104 80-81 -0.8367255476538489 1104 82-83 -0.8505226439582252 1104 84-85 -0.8316953098807787 1104 86-87 -0.8541184076040658 1104 88-89 -0.928666743491565 1104 90-91 -0.9859504126881937 1104 92-93 -1.0439167577470485 1104 94-95 -1.083699002586723 1104 96-97 -1.1730088404672685 1104 98-99 -1.1294073191681164 1104 100 -1.0492141468856033 1105 1 -0.4897317614114982 1105 2 -0.4592923379879359 1105 3 -0.4723925181329989 1105 4 -0.33269887583376345 1105 5 -0.26670287018619376 1105 6 -0.2678022332949226 1105 7 -0.30451325465174506 1105 8 -0.38062276149084084 1105 9 -0.5572465741700725 1105 10-11 -0.533006224061026 1105 12-13 -0.4959851565435258 1105 14-15 -0.7278310073597325 1105 16-17 -0.6760609817552705 1105 18-19 -0.6896730417395247 1105 20-21 -0.6923549066509835 1105 22-23 -0.6556104097178448 1105 24-25 -0.7308317792874064 1105 26-27 -0.7362122256832677 1105 28-29 -0.7399025618010171 1105 30-31 -0.7258652980667364 1105 32-33 -0.8133300218132078 1105 34-35 -0.7760184495423843 1105 36-37 -0.787133882987959 1105 38-39 -0.8437583720758681 1105 40-41 -0.8702474664004782 1105 42-43 -0.8816811948506071 1105 44-45 -0.8869803262454425 1105 46-47 -0.9004320799082066 1105 48-49 -0.9904206794940436 1105 50-51 -0.9612783204499067 1105 52-53 -0.8309783033656473 1105 54-55 -0.7077128058833324 1105 56-57 -0.6306176622377322 1105 58-59 -0.5746730776988969 1105 60-61 -0.5687050277095551 1105 62-63 -0.6296608423517256 1105 64-65 -0.678419891887998 1105 66-67 -0.6493296835942033 1105 68-69 -0.6094862147979043 1105 70-71 -0.5823840912835863 1105 72-73 -0.6034937480616804 1105 74-75 -0.7882576656188363 1105 76-77 -0.7263562692081038 1105 78-79 -0.7013639708397363 1105 80-81 -0.7116343606504714 1105 82-83 -0.8156128359633676 1105 84-85 -0.8333944513978544 1105 86-87 -0.8147449088557224 1105 88-89 -0.8722478279970574 1105 90-91 -0.9302201095103193 1105 92-93 -0.9781382243705679 1105 94-95 -0.9877933235321237 1105 96-97 -0.9876426601933765 1105 98-99 -0.9543316655555216 1105 100 -1.0051396080919552 1106 1 -0.4684601141363487 1106 2 -0.44211675779045123 1106 3 -0.4664899417815569 1106 4 -0.33315747219364766 1106 5 -0.3289917245146299 1106 6 -0.37735455025794806 1106 7 -0.3367261701773856 1106 8 -0.3873506129980768 1106 9 -0.4664438262533963 1106 10-11 -0.44507464628283344 1106 12-13 -0.5266789824351079 1106 14-15 -0.6619756869888604 1106 16-17 -0.6477923822857719 1106 18-19 -0.7458738359981254 1106 20-21 -0.6501031935666433 1106 22-23 -0.6337959150408494 1106 24-25 -0.6542467232663967 1106 26-27 -0.6799660317800047 1106 28-29 -0.770349318168762 1106 30-31 -0.775710626273252 1106 32-33 -0.7814168038911262 1106 34-35 -0.7838638320947453 1106 36-37 -0.8422048278874854 1106 38-39 -0.7958479299189563 1106 40-41 -0.8127070079445389 1106 42-43 -0.8369335158267432 1106 44-45 -0.8671471836586377 1106 46-47 -0.7318268956815075 1106 48-49 -0.7442478851031424 1106 50-51 -0.7499433607302208 1106 52-53 -0.6987245500641492 1106 54-55 -0.6343787932846254 1106 56-57 -0.5629683699505179 1106 58-59 -0.5819401969762694 1106 60-61 -0.6086869833683011 1106 62-63 -0.6355235470243699 1106 64-65 -0.6432099311552335 1106 66-67 -0.6180255391427281 1106 68-69 -0.701772192624226 1106 70-71 -0.6384471705966916 1106 72-73 -0.5898976035867207 1106 74-75 -0.6247624661816431 1106 76-77 -0.5244400410520811 1106 78-79 -0.634325256506024 1106 80-81 -0.6656961226360458 1106 82-83 -0.6863155471810352 1106 84-85 -0.6701579653750827 1106 86-87 -0.6984850941367675 1106 88-89 -0.726127359636429 1106 90-91 -0.753053005373463 1106 92-93 -0.7929348470474231 1106 94-95 -1.0119550132713897 1106 96-97 -0.9452400953186633 1106 98-99 -0.8235549570673406 1106 100 -1.0965452789687191 1107 1 -0.6335279653913446 1107 2 -0.5727296273820102 1107 3 -0.5926263074969 1107 4 -0.469446513785428 1107 5 -0.488283773079651 1107 6 -0.4889364261746678 1107 7 -0.528539891117596 1107 8 -0.5407685727568463 1107 9 -0.674650642019742 1107 10-11 -0.6481075708974373 1107 12-13 -0.6818346160425435 1107 14-15 -0.8669858068880032 1107 16-17 -0.8474931287246363 1107 18-19 -0.8593259695243205 1107 20-21 -0.8402548453630274 1107 22-23 -0.8967484171895777 1107 24-25 -0.8816204723915178 1107 26-27 -0.9109232290029468 1107 28-29 -1.053258459145674 1107 30-31 -0.9486526193680049 1107 32-33 -0.9470843955288188 1107 34-35 -1.017476672127735 1107 36-37 -1.0410394981860662 1107 38-39 -1.0524266744122386 1107 40-41 -1.0363184696795074 1107 42-43 -1.0193774002183744 1107 44-45 -1.0600615371721815 1107 46-47 -0.9983795924803971 1107 48-49 -1.0405157602430606 1107 50-51 -1.0659351202141494 1107 52-53 -0.9618356898399085 1107 54-55 -0.8879883723963147 1107 56-57 -0.8207326094409169 1107 58-59 -0.8789860676732246 1107 60-61 -0.8754964055307966 1107 62-63 -0.9008540265409337 1107 64-65 -0.9108879643096373 1107 66-67 -0.8953318039050728 1107 68-69 -0.8459072557344243 1107 70-71 -0.9251570791134469 1107 72-73 -0.8007243538185591 1107 74-75 -0.817517460186501 1107 76-77 -0.876161696842729 1107 78-79 -0.7908366830550513 1107 80-81 -0.8343580904078536 1107 82-83 -0.7995717642297571 1107 84-85 -0.9006531638481263 1107 86-87 -0.8693358181135338 1107 88-89 -0.9433056655298451 1107 90-91 -0.9195224918105396 1107 92-93 -0.9376229817177837 1107 94-95 -0.997126401312272 1107 96-97 -0.984067646431356 1107 98-99 -0.9592052987091328 1107 100 -0.9125355726471511 1108 1 -0.5502550197717078 1108 2 -0.5255513749638254 1108 3 -0.5547011994968116 1108 4 -0.33914075534295307 1108 5 -0.3281904582628812 1108 6 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1.066594527705373 2208 84-85 1.0560209726657988 2208 86-87 1.0819184718559107 2208 88-89 1.1095731790236236 2208 90-91 1.1442624034962385 2208 92-93 1.1507274554355682 2208 94-95 1.126283072653873 2208 96-97 1.1614276710029259 2208 98-99 1.2556437356129244 2208 100 1.3691663345979883 2209 1 0.6905753377584389 2209 2 0.6668961971018739 2209 3 0.6784818508465129 2209 4 0.5285704281000321 2209 5 0.46780724841109134 2209 6 0.525110068687539 2209 7 0.5823852226286235 2209 8 0.5775579093304728 2209 9 0.7349316138103958 2209 10-11 0.7240277058121904 2209 12-13 0.767063704093907 2209 14-15 0.9360009072868039 2209 16-17 0.9777146048708119 2209 18-19 0.9304775659424962 2209 20-21 0.9580707568213995 2209 22-23 0.9483272863395413 2209 24-25 0.9951612069817912 2209 26-27 0.9534412281911955 2209 28-29 1.0178433886220546 2209 30-31 1.0105012541104514 2209 32-33 1.0826147977660057 2209 34-35 1.1051868739772672 2209 36-37 1.112220669545252 2209 38-39 1.1468950370003057 2209 40-41 1.1662618845118615 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0.8885423788246882 2210 70-71 0.8505818970664194 2210 72-73 0.8532175627787666 2210 74-75 0.8682125487232426 2210 76-77 0.8534303163342472 2210 78-79 0.9060937198951464 2210 80-81 0.9601336273085153 2210 82-83 1.0072449864809165 2210 84-85 1.0067618203042343 2210 86-87 1.0427699050337473 2210 88-89 1.0154905431686565 2210 90-91 1.02907874826818 2210 92-93 1.0915268210223168 2210 94-95 1.124116472435901 2210 96-97 1.1500780997280522 2210 98-99 1.192847743360339 2210 100 1.3808507758541388 2211 1 0.6422108228745742 2211 2 0.6165983375406121 2211 3 0.6489816312821297 2211 4 0.5464472394228181 2211 5 0.48514820816584603 2211 6 0.5623597689051891 2211 7 0.6112408226206654 2211 8 0.49659971225468524 2211 9 0.6933503657088664 2211 10-11 0.7227713304705787 2211 12-13 0.7648445881220098 2211 14-15 0.893409849197667 2211 16-17 0.9285258621939292 2211 18-19 0.9035662791218328 2211 20-21 0.9305723742401071 2211 22-23 0.9877125849843296 2211 24-25 0.9343601565404427 2211 26-27 1.0093989417918792 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1.077005301225057 2211 96-97 1.1084613634312994 2211 98-99 1.1822139264710039 2211 100 1.249085154615237 2212 1 0.6576103872861978 2212 2 0.6565809419977633 2212 3 0.6656151913516517 2212 4 0.5235487630278612 2212 5 0.5320054238515439 2212 6 0.5423911574371161 2212 7 0.614955705686647 2212 8 0.6040769911426338 2212 9 0.7553386738173771 2212 10-11 0.701388814242307 2212 12-13 0.7915966899199702 2212 14-15 0.9825600181881597 2212 16-17 0.9738501526583576 2212 18-19 0.9612559604931903 2212 20-21 0.958723403836018 2212 22-23 0.992160451186038 2212 24-25 1.050894952158984 2212 26-27 1.080506258326885 2212 28-29 1.0683602276904125 2212 30-31 1.013260465561693 2212 32-33 1.0626432921400024 2212 34-35 1.1002275916899862 2212 36-37 1.1314837198286511 2212 38-39 1.1918901998266946 2212 40-41 1.1923122414357366 2212 42-43 1.2359682126479328 2212 44-45 1.1900096995919967 2212 46-47 1.188286716876 2212 48-49 1.2281375340754224 2212 50-51 1.276799280674858 2212 52-53 1.1359499315666994 2212 54-55 1.067065360670341 2212 56-57 1.0013759602539452 2212 58-59 1.0383229098284588 2212 60-61 1.000110103873837 2212 62-63 0.9666272422115227 2212 64-65 1.0292162792725108 2212 66-67 1.0190961736649484 2212 68-69 0.9955687078391193 2212 70-71 1.027364192055913 2212 72-73 1.0522814050723 2212 74-75 1.0561816831677575 2212 76-77 1.0589042691443566 2212 78-79 0.9971732165572078 2212 80-81 1.0103671381056998 2212 82-83 1.0931616279223455 2212 84-85 1.0289287033810126 2212 86-87 1.038892009561895 2212 88-89 1.0798329935198474 2212 90-91 1.1515139949977176 2212 92-93 1.156959308606364 2212 94-95 1.22982836185556 2212 96-97 1.2499754789133775 2212 98-99 1.271168533445561 2212 100 1.3563928094652304 2213 1 0.6275263546361458 2213 2 0.5809432356114748 2213 3 0.6104323492328412 2213 4 0.47648469528133575 2213 5 0.447963834156198 2213 6 0.5304983012557614 2213 7 0.5490418390241345 2213 8 0.5095565031926697 2213 9 0.5309942659315823 2213 10-11 0.6128892857634298 2213 12-13 0.7134488436720474 2213 14-15 0.8979080144262994 2213 16-17 0.9030955204995763 2213 18-19 0.8826004674364256 2213 20-21 0.8840434952470915 2213 22-23 0.9163026801431968 2213 24-25 0.9595146009804196 2213 26-27 0.9433354262457172 2213 28-29 0.9665835862320833 2213 30-31 0.9844960222084396 2213 32-33 0.983724581968282 2213 34-35 1.0145595439729007 2213 36-37 1.060090048065824 2213 38-39 1.0656759186300704 2213 40-41 1.1105136650031469 2213 42-43 1.1295523051317673 2213 44-45 1.095516423453489 2213 46-47 1.0611853757104726 2213 48-49 1.1131264587071357 2213 50-51 1.1408746400672172 2213 52-53 1.0367365493581602 2213 54-55 0.9437514173896773 2213 56-57 0.9267093387578242 2213 58-59 0.875265661844935 2213 60-61 0.8956575398256064 2213 62-63 0.8944060640927276 2213 64-65 0.9236041217178652 2213 66-67 0.9035701606545459 2213 68-69 0.9176429486166953 2213 70-71 0.8918841183798492 2213 72-73 0.9217984247239315 2213 74-75 0.9155300514187203 2213 76-77 0.9387354863541191 2213 78-79 0.9598108390860389 2213 80-81 0.8161367248455562 2213 82-83 1.0129573577096345 2213 84-85 1.023933284626338 2213 86-87 0.9929714658615261 2213 88-89 1.0198472602378494 2213 90-91 1.1248287208192274 2213 92-93 1.1792381467824562 2213 94-95 1.2275882951972505 2213 96-97 1.2667448688264997 2213 98-99 1.2844952234471876 2213 100 1.2994833215535238 2214 1 0.5961286774234864 2214 2 0.5318443169090266 2214 3 0.5798356331735697 2214 4 0.4424438462625133 2214 5 0.4584564252655241 2214 6 0.4866857261656534 2214 7 0.5027462843585369 2214 8 0.506673043040486 2214 9 0.6487355554790355 2214 10-11 0.6618680602928606 2214 12-13 0.7035169253700886 2214 14-15 0.829986508619335 2214 16-17 0.8425428573037408 2214 18-19 0.8288960221020218 2214 20-21 0.8596942761842215 2214 22-23 0.8653215027303034 2214 24-25 0.8754537279327579 2214 26-27 0.9117775568023845 2214 28-29 0.8495631617226707 2214 30-31 0.8966706317398803 2214 32-33 0.9484021951040447 2214 34-35 0.9906508535443876 2214 36-37 0.9326971766200955 2214 38-39 0.9112105601999545 2214 40-41 1.025491638571424 2214 42-43 1.1126920728530294 2214 44-45 1.1308388103177265 2214 46-47 1.1800880589303446 2214 48-49 1.1949446405253141 2214 50-51 1.157774920403618 2214 52-53 1.1143897331787471 2214 54-55 0.9778723625238328 2214 56-57 0.9434894749212148 2214 58-59 0.9219216490295565 2214 60-61 0.9234508918480344 2214 62-63 0.9426639735841178 2214 64-65 0.9632116507527044 2214 66-67 0.9558328759128543 2214 68-69 0.9504823559054429 2214 70-71 0.9620483154162969 2214 72-73 0.965851288160053 2214 74-75 1.0053578447707494 2214 76-77 1.0202332837109438 2214 78-79 1.0454335863827922 2214 80-81 1.0024402891226813 2214 82-83 1.0243710541453588 2214 84-85 1.0257354472214502 2214 86-87 1.0659790749924873 2214 88-89 1.1188460587961195 2214 90-91 1.1513405349962405 2214 92-93 1.2115168812638295 2214 94-95 1.2392422799789884 2214 96-97 1.2589354975810068 2214 98-99 1.3294691922097037 2214 100 1.359235023595975 2215 1 0.552950863561378 2215 2 0.5230946960758978 2215 3 0.5517656614819977 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66-67 0.9151206439385859 2215 68-69 0.9540597339601717 2215 70-71 0.9279242919056685 2215 72-73 0.912907901739203 2215 74-75 0.9436135355627968 2215 76-77 0.9534447403393926 2215 78-79 1.003122173477017 2215 80-81 1.0482416055855168 2215 82-83 1.0718567772023668 2215 84-85 1.0830309288809161 2215 86-87 1.0647493705260267 2215 88-89 1.0535900090005619 2215 90-91 1.1259839836618717 2215 92-93 1.1356426944453268 2215 94-95 1.057173842355823 2215 96-97 1.1881867978629224 2215 98-99 1.2810084306705605 2215 100 1.2823538155840168 2216 1 0.40560110382988057 2216 2 0.37408157048047386 2216 3 0.3780846158974249 2216 4 0.24653788706584123 2216 5 0.22682942641213089 2216 6 0.30306022478975336 2216 7 0.3239658146245219 2216 8 0.2649632684095451 2216 9 0.32767210492924903 2216 10-11 0.27740407922870247 2216 12-13 0.4202191685509078 2216 14-15 0.5557676497376534 2216 16-17 0.4522805082550505 2216 18-19 0.5463021090804006 2216 20-21 0.5632447306986634 2216 22-23 0.6071663375589864 2216 24-25 0.5534758140938507 2216 26-27 0.49937328292067207 2216 28-29 0.6073188744459088 2216 30-31 0.700501781687052 2216 32-33 0.6948785698507223 2216 34-35 0.6833811029488075 2216 36-37 0.681663515689074 2216 38-39 0.4801332348096494 2216 40-41 0.7233666925481756 2216 42-43 0.8041202218396393 2216 44-45 0.7626745029614526 2216 46-47 0.7408486325804162 2216 48-49 0.5467387412864966 2216 50-51 0.771216021199713 2216 52-53 0.622645916066837 2216 54-55 0.483770083635946 2216 56-57 0.5089734183264838 2216 58-59 0.5283014155888779 2216 60-61 0.5618119210080437 2216 62-63 0.2650373579126324 2216 64-65 0.39104328832659263 2216 66-67 0.4937952359608886 2216 68-69 0.6216850281259596 2216 70-71 0.44115476720081404 2216 72-73 0.5589523656062028 2216 74-75 0.5836876514597193 2216 76-77 0.6191000411793226 2216 78-79 0.7056722950010297 2216 80-81 0.6103047223675944 2216 82-83 0.5031015046060467 2216 84-85 0.5522543114232832 2216 86-87 0.7459796233277523 2216 88-89 0.8012034043181693 2216 90-91 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1.033294254703467 2310 84-85 1.028218443462798 2310 86-87 0.9714200111070816 2310 88-89 0.9910258247441384 2310 90-91 1.0962365575391466 2310 92-93 1.1408957230566692 2310 94-95 1.1253215884658623 2310 96-97 1.1294636769643063 2310 98-99 1.2066640482591815 2310 100 1.355230651141369 2311 1 0.6439100424909014 2311 2 0.6133564817326054 2311 3 0.6560354388879155 2311 4 0.481821690074824 2311 5 0.4761243302135796 2311 6 0.5150634422448164 2311 7 0.5349149352426892 2311 8 0.4759093629515192 2311 9 0.6734778202057399 2311 10-11 0.7019727304697483 2311 12-13 0.7704189304723172 2311 14-15 0.9627487870597591 2311 16-17 0.9946945646243037 2311 18-19 0.886830695072689 2311 20-21 0.9284567543625784 2311 22-23 0.9807920593701667 2311 24-25 0.9971727427491928 2311 26-27 1.0401527878791441 2311 28-29 1.0430159004264041 2311 30-31 1.0600762049639627 2311 32-33 1.0990316140629233 2311 34-35 1.136443369373545 2311 36-37 1.1629122505643181 2311 38-39 1.1736006314967469 2311 40-41 1.2106647377438478 2311 42-43 1.236097995718957 2311 44-45 1.2681186389369614 2311 46-47 1.2376093406986044 2311 48-49 1.2637008593108234 2311 50-51 1.2976437464369042 2311 52-53 1.152993595863812 2311 54-55 1.069031681788573 2311 56-57 1.0367979026360743 2311 58-59 0.9915826799652905 2311 60-61 0.9871389314778583 2311 62-63 1.0145717715551612 2311 64-65 1.0033270312229874 2311 66-67 0.99633300977478 2311 68-69 0.9973721007693754 2311 70-71 0.9958807825822831 2311 72-73 0.9635469306915851 2311 74-75 0.9942481257521791 2311 76-77 1.0272285520922182 2311 78-79 1.039651511582612 2311 80-81 0.9903900536268466 2311 82-83 1.0144634801594385 2311 84-85 1.0360178178440833 2311 86-87 1.08027494219235 2311 88-89 1.1231788513902465 2311 90-91 1.133757424894494 2311 92-93 1.1881435916370684 2311 94-95 1.2226080857966153 2311 96-97 1.2262463927492036 2311 98-99 1.3246492185775196 2311 100 1.420148521198012 2312 1 0.521419356072947 2312 2 0.5410669449488985 2312 3 0.5591613554412582 2312 4 0.27345238417751005 2312 5 0.3749589069041619 2312 6 0.4445886863128692 2312 7 0.33892516461537525 2312 8 0.4311072950107473 2312 9 0.6319788757492191 2312 10-11 0.5571066077525444 2312 12-13 0.7146141267360306 2312 14-15 0.9160745785878177 2312 16-17 0.9099174599503499 2312 18-19 0.8596034657370382 2312 20-21 0.8643714696926494 2312 22-23 0.9647502391697103 2312 24-25 0.9424992933892469 2312 26-27 0.9741554721031775 2312 28-29 0.99855412479139 2312 30-31 1.000071230866297 2312 32-33 0.9950232611562555 2312 34-35 0.9940488741976523 2312 36-37 1.0302936627182504 2312 38-39 1.0543801781676407 2312 40-41 1.0750031196484002 2312 42-43 1.118040136372997 2312 44-45 1.1523361513548522 2312 46-47 1.1411927555474577 2312 48-49 1.2166682834428784 2312 50-51 1.2760517451470363 2312 52-53 1.0900043721281278 2312 54-55 0.9886531555474249 2312 56-57 0.9616347608418607 2312 58-59 0.9230148982933244 2312 60-61 0.8786994022877792 2312 62-63 0.8542540169017983 2312 64-65 0.89664676205836 2312 66-67 0.9281249537425822 2312 68-69 0.9055054650957359 2312 70-71 0.8822999036790335 2312 72-73 0.8886853190154014 2312 74-75 0.9413280855214126 2312 76-77 0.8871833589336475 2312 78-79 0.9171720463478437 2312 80-81 0.9147817422113818 2312 82-83 0.9363338085844539 2312 84-85 0.965472600404297 2312 86-87 1.0101202302858816 2312 88-89 1.0039770582410021 2312 90-91 1.0607017968296155 2312 92-93 1.090330961741948 2312 94-95 1.1396743059897467 2312 96-97 1.1716289239288358 2312 98-99 0.8468772749791569 2312 100 1.1689328512273462 2313 1 0.5702684407611258 2313 2 0.5301015623132024 2313 3 0.5737981447632059 2313 4 0.4460744405185082 2313 5 0.31799359769469504 2313 6 0.46689923772996167 2313 7 0.49738174202290963 2313 8 0.5128556165151892 2313 9 0.6648478358125587 2313 10-11 0.6904676011108961 2313 12-13 0.7594834513178697 2313 14-15 0.9262296396511971 2313 16-17 0.9042463247615302 2313 18-19 0.8914514120465142 2313 20-21 0.9432421729349443 2313 22-23 0.969325628053177 2313 24-25 0.9510128713767116 2313 26-27 0.9526478329586396 2313 28-29 1.012112224846085 2313 30-31 1.0090612560845855 2313 32-33 1.0125334640137638 2313 34-35 1.0548780617930404 2313 36-37 1.0965727883082081 2313 38-39 1.1104123306368479 2313 40-41 1.1399315346545293 2313 42-43 1.2025993244040976 2313 44-45 1.1318221272037263 2313 46-47 1.1311996853178812 2313 48-49 1.1465951949530861 2313 50-51 1.1408332900060358 2313 52-53 1.091489810860594 2313 54-55 0.9625489065773181 2313 56-57 0.8924756492796391 2313 58-59 0.9163229194447595 2313 60-61 0.8738052298129446 2313 62-63 0.9289544818411386 2313 64-65 0.9237374004237537 2313 66-67 0.9178203731460997 2313 68-69 0.8964047426257409 2313 70-71 0.9155984602494698 2313 72-73 0.890890745736229 2313 74-75 0.8920526905445101 2313 76-77 0.6883292449026044 2313 78-79 0.8899062314526489 2313 80-81 0.9312834910646686 2313 82-83 0.9830738418788094 2313 84-85 0.993067472575575 2313 86-87 1.0208163811485313 2313 88-89 1.0830559975057774 2313 90-91 1.1348702822106311 2313 92-93 1.2270769845264518 2313 94-95 1.2090828571734988 2313 96-97 1.1964320209372161 2313 98-99 1.2229622203080517 2313 100 1.427304427841694 2314 1 0.5027078082619809 2314 2 0.5199080749377352 2314 3 0.5213395940897065 2314 4 0.4335505421155119 2314 5 0.42705407392205785 2314 6 0.4566196517871859 2314 7 0.4790584318607287 2314 8 0.4852597353508443 2314 9 0.6759307498694511 2314 10-11 0.6531542283466791 2314 12-13 0.6882143226003379 2314 14-15 0.8313744108018994 2314 16-17 0.8363748401978626 2314 18-19 0.8028981263131101 2314 20-21 0.8470364775608488 2314 22-23 0.8672241263599076 2314 24-25 0.8680694174231007 2314 26-27 0.9425464880318941 2314 28-29 0.8959649299470058 2314 30-31 0.9553892558173658 2314 32-33 0.9554894459173155 2314 34-35 0.9116947737883763 2314 36-37 0.9372600722946061 2314 38-39 0.9458325190567294 2314 40-41 1.0221077579769187 2314 42-43 1.0647001069824427 2314 44-45 1.1889117618962857 2314 46-47 1.162443336400976 2314 48-49 1.0908394261685643 2314 50-51 1.105074846135416 2314 52-53 1.0058700874398383 2314 54-55 0.9269830779982158 2314 56-57 0.9018053764125611 2314 58-59 0.8764887533884789 2314 60-61 0.8943071519678085 2314 62-63 0.953351491681623 2314 64-65 0.9447303210863893 2314 66-67 0.9339702059405184 2314 68-69 0.9100773729542198 2314 70-71 0.9148740569363412 2314 72-73 0.9360209230527516 2314 74-75 0.9243801018278859 2314 76-77 0.8970219696659356 2314 78-79 0.8904710000793585 2314 80-81 0.8899271427200546 2314 82-83 0.9414556610919327 2314 84-85 1.00317159207178 2314 86-87 1.0146133798479546 2314 88-89 1.0061084951143648 2314 90-91 1.1093816297183245 2314 92-93 1.121073843152228 2314 94-95 1.0908578510099431 2314 96-97 1.168064294664923 2314 98-99 1.228333352200547 2314 100 1.278607375017451 2315 1 0.4099045922288411 2315 2 0.45733425247964377 2315 3 0.45770534957543063 2315 4 0.17819253718465689 2315 5 0.25405611456979216 2315 6 0.34839462358899453 2315 7 0.3655079776725998 2315 8 0.354853311419582 2315 9 0.49982175319592415 2315 10-11 0.4091483107714424 2315 12-13 0.4281762592358618 2315 14-15 0.6488651111475789 2315 16-17 0.6588557059560145 2315 18-19 0.5201811223275215 2315 20-21 0.6330141794255937 2315 22-23 0.48143072725981995 2315 24-25 0.6096203236515834 2315 26-27 0.6903932527921413 2315 28-29 0.7289636086251221 2315 30-31 0.721886407799488 2315 32-33 0.7649014073697131 2315 34-35 0.7651559653005222 2315 36-37 0.86015215176581 2315 38-39 0.8414077770273352 2315 40-41 0.8874905545628664 2315 42-43 0.9402990678262455 2315 44-45 0.9210535587550694 2315 46-47 0.9599756959354266 2315 48-49 0.9840502592855316 2315 50-51 0.9375473987298477 2315 52-53 0.9233340739930327 2315 54-55 0.8059839590851681 2315 56-57 0.7924770979642872 2315 58-59 0.8298761838173121 2315 60-61 0.7755552823846656 2315 62-63 0.784331013861987 2315 64-65 0.8088507021579119 2315 66-67 0.758108667016252 2315 68-69 0.7799145767176086 2315 70-71 0.7780594284404998 2315 72-73 0.5139307537203308 2315 74-75 0.7116828483137638 2315 76-77 0.7691230643800537 2315 78-79 0.8138941491548266 2315 80-81 0.5083211537327443 2315 82-83 0.5180283667034153 2315 84-85 0.8113557493614323 2315 86-87 0.9063968502122712 2315 88-89 0.9586713905809212 2315 90-91 1.0114177940732993 2315 92-93 1.0788709006453594 2315 94-95 1.033036153722385 2315 96-97 0.9690653488623013 2315 98-99 0.4227415583212881 2315 100 0.9233732975069628 2316 1 0.3401588347491611 2316 2 0.2723871990111313 2316 3 0.339669446163402 2316 4 0.20778097018397546 2316 5 0.17788074719277347 2316 6 0.18978797221929966 2316 7 0.15461436074474477 2316 8 0.2109197464023893 2316 9 0.28749014331047107 2316 10-11 0.25806461998023167 2316 12-13 0.135033150590111 2316 14-15 0.10750169934085108 2316 16-17 0.3602298935734609 2316 18-19 0.38075531630636306 2316 20-21 0.3744733962966009 2316 22-23 0.3320881794231454 2316 24-25 0.41020991428483455 2316 26-27 0.46703867903504914 2316 28-29 0.4176413795958709 2316 30-31 0.36320249872903787 2316 32-33 0.523514786927727 2316 34-35 0.518669261651759 2316 36-37 0.47698688748776874 2316 38-39 0.6589724154259002 2316 40-41 0.7008870428180387 2316 42-43 0.6374371540788886 2316 44-45 0.5697745858455505 2316 46-47 0.4501978685849721 2316 48-49 0.3614978954799426 2316 50-51 0.4950639109034256 2316 52-53 0.5816965909212684 2316 54-55 0.28739538440032675 2316 56-57 0.12068612920102595 2316 58-59 0.19745172975236613 2316 60-61 0.29529063433156466 2316 62-63 0.43386789067927367 2316 64-65 0.40668148524995473 2316 66-67 0.3259398693817914 2316 68-69 0.3371202481974933 2316 70-71 0.057677238696598465 2316 72-73 0.0026400344596027026 2316 74-75 0.2647183750222055 2316 76-77 0.44436752411991876 2316 78-79 0.4949923937696923 2316 80-81 0.6456047359197967 2316 82-83 0.6521463871585063 2316 84-85 0.6730811382349202 2316 86-87 0.6950635516666885 2316 88-89 0.6881084353271483 2316 90-91 0.5737479662201501 2316 92-93 0.555347609467308 2316 94-95 0.7759487583312534 2316 96-97 0.7382558862290232 2316 98-99 0.5319290306541866 2316 100 0.47817819316489363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 24.0 1 619.0 2 232.0 3 212.0 4 162.0 5 139.0 6 165.0 7 706.0 8 2374.0 9 4783.0 10 7060.0 11 9533.0 12 12191.0 13 14305.0 14 16507.0 15 19394.0 16 24138.0 17 31501.0 18 40554.0 19 51913.0 20 67455.0 21 86201.0 22 95523.0 23 92997.0 24 87304.0 25 87109.0 26 95877.0 27 113153.0 28 135852.0 29 167153.0 30 208261.0 31 265987.0 32 347519.0 33 469183.0 34 699588.0 35 985459.0 36 1511158.0 37 2088643.0 38 1808124.0 39 570195.0 40 474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88657345150869 21.229239364036772 13.018583276196924 22.86560390825761 2 15.52166863733255 19.9558922256741 41.5875722245454 22.93486691244796 3 19.12429748154713 27.03210071501838 28.538707791763585 25.304894011670903 4 12.279840537947106 16.39931278842799 39.94128069735044 31.379565976274463 5 12.892915846407323 38.65684459627092 34.60546565529485 13.844773902026903 6 29.264316039458333 38.48354333173887 18.18733172242421 14.064808906378584 7 27.064611444314558 32.18290029939357 23.235604534177668 17.5168837221142 8 26.291076181289736 35.09828013796802 19.99308652291947 18.617557157822777 9 26.50984456628044 15.39470916293166 22.065358573737754 36.03008769705015 10-11 25.73653213999586 26.37264782339527 27.131377130816723 20.759442905792145 12-13 25.796571674467067 24.1297771003031 27.879558319749687 22.194092905480144 14-15 23.191196382407444 25.208104057166654 25.980062332125804 25.620637228300097 16-17 21.474375128633508 27.99590682427246 27.175035425288925 23.354682621805107 18-19 20.861400882082066 27.90412609298107 29.76086230712743 21.47361071780944 20-21 22.49643778203033 27.073957605324356 29.130100450680885 21.29950416196443 22-23 22.803852263256292 26.809684772694165 28.774320045840284 21.612142918209262 24-25 22.105634817732337 26.79338447034436 29.711428801430117 21.389551910493186 26-27 22.016310589307007 27.087137443572345 28.72932397016767 22.167227996952978 28-29 21.242042047097048 27.46862547310251 29.285814739453407 22.003517740347036 30-31 22.221044574158626 27.11684889954905 29.170497488942793 21.491609037349534 32-33 22.44080770743263 27.328856226722447 28.300110144769807 21.930225921075117 34-35 22.420186060800884 27.17425596648641 29.137648750793556 21.26790922191915 36-37 22.007236712715347 26.829416770830434 28.77228734724286 22.391059169211367 38-39 22.254583342528996 27.092589858587758 29.148785690527873 21.504041108355377 40-41 22.028413261361983 26.11514101098954 29.712522571384152 22.143923156264325 42-43 21.94187661306489 26.362051239084405 29.02594274246088 22.67012940538983 44-45 22.005460830634373 27.277801477770115 28.908909672226823 21.807828019368692 46-47 22.0525633902559 27.030593165953594 28.253423090216025 22.66342035357448 48-49 22.73418626794269 27.017771216692122 28.1244160387416 22.12362647662359 50-51 22.427265850577943 26.7660015764924 28.145199971165663 22.661532601763994 52-53 21.90569235444517 27.42208315368828 27.879851952082014 22.792372539784537 54-55 21.846573727764987 27.336191478393374 27.949137762562525 22.868097031279113 56-57 22.764559302878812 26.919239955326496 27.46605915612228 22.85014158567241 58-59 21.844310731279318 27.26761333551436 28.322060645893522 22.56601528731281 60-61 21.96650930118844 28.003629884851378 27.571685527948198 22.458175286011986 62-63 22.278735473841046 27.201986733901194 27.32940458762998 23.18987320462778 64-65 21.609534699182728 27.249867441764298 27.734985317661405 23.40561254139157 66-67 22.13515795851379 26.916205139172778 27.60534829471168 23.34328860760175 68-69 21.690564117668853 26.995963180114877 27.912544819497686 23.400927882718584 70-71 21.791442234054482 26.63099336083586 27.972320166810427 23.605244238299235 72-73 22.409184981583234 26.765935887541847 28.085992122882136 22.738887007992776 74-75 22.571639787656242 27.039141949245852 27.62161620934797 22.76760205374993 76-77 22.245274348164397 26.950483645259638 27.41718031552122 23.387061691054743 78-79 22.07452360500068 27.001998193532046 27.446857320251887 23.476620881215386 80-81 21.758076599548833 27.405783506265045 27.901561271765154 22.93457862242097 82-83 22.740835696153944 26.454777663890006 28.840989146146068 21.963397493809982 84-85 22.76989166959682 26.918755182409566 27.629777125929238 22.68157602206438 86-87 21.600022234709673 27.551437828248222 28.049140078475215 22.79939985856689 88-89 22.65694638929156 26.393453869705315 27.89692562569706 23.052674115306065 90-91 22.388451765949867 26.486010970350616 28.005022494694586 23.12051476900493 92-93 22.724169846337418 26.645756704222457 27.885052688074914 22.745020761365218 94-95 21.627409307940717 26.681691681987747 28.4859854336791 23.204913576392443 96-97 22.012335164281264 26.60246440985255 28.00221859134131 23.382981834524873 98-99 21.488938345751627 27.0191953408562 27.843450286138733 23.648416027253443 100 22.332429916695787 26.450580760847114 27.04976230256725 24.167227019889847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1456.0 1 980.5 2 1053.5 3 1485.0 4 1126.0 5 961.0 6 1394.0 7 2538.0 8 4067.0 9 4972.0 10 4827.5 11 4742.0 12 5403.5 13 6422.5 14 7693.5 15 9239.5 16 10528.0 17 11650.5 18 12948.0 19 13883.5 20 14800.5 21 16067.5 22 17758.5 23 19941.5 24 22463.0 25 26132.5 26 32921.0 27 40741.0 28 50228.0 29 69028.0 30 96771.5 31 123840.5 32 162007.0 33 204291.0 34 236760.0 35 268302.0 36 297338.5 37 343425.5 38 360471.0 39 340542.5 40 316763.0 41 287309.5 42 277045.0 43 287901.0 44 311162.5 45 361260.0 46 404571.0 47 429959.5 48 448898.0 49 442377.0 50 426089.0 51 438234.0 52 440274.0 53 413490.0 54 385670.5 55 328704.0 56 282856.5 57 232141.0 58 181587.5 59 171496.5 60 141522.0 61 97221.5 62 72246.5 63 60724.0 64 44952.0 65 23165.5 66 13761.0 67 9605.0 68 7401.5 69 5915.0 70 4816.5 71 3843.5 72 3147.5 73 2941.5 74 2537.0 75 1975.5 76 1478.5 77 1069.5 78 779.0 79 568.5 80 416.0 81 328.0 82 261.5 83 195.5 84 144.5 85 95.5 86 71.5 87 62.5 88 46.0 89 27.0 90 23.0 91 23.0 92 27.0 93 25.0 94 14.5 95 10.0 96 8.5 97 6.5 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057398793529416195 2 0.13075691747930251 3 0.016321375316581354 4 0.01587126544574038 5 0.018806764603398897 6 0.05093091038537526 7 0.05623437886354499 8 0.047907346252986996 9 0.03904213879685827 10-11 0.038127241559388035 12-13 0.03924762373789437 14-15 0.018415364715711095 16-17 0.017065035103188176 18-19 0.01706014260459208 20-21 0.020313654170996936 22-23 0.024584805445390077 24-25 0.02352802574863301 26-27 0.01845939720307597 28-29 0.01765213493471988 30-31 0.015856587949952088 32-33 0.022163018640321802 34-35 0.02211898615295692 36-37 0.02194285620349741 38-39 0.020034781751019377 40-41 0.02368458570370813 42-43 0.030832526152606617 44-45 0.039776013586272904 46-47 0.045514914439495305 48-49 0.03853331894286413 50-51 0.026825569802402742 52-53 0.03894918132353242 54-55 0.035817982222030004 56-57 0.037452076753126576 58-59 0.042349467847820205 60-61 0.022730548477469113 62-63 0.02501045282325056 64-65 0.02465330042573544 66-67 0.03130709851642808 68-69 0.03804896158185047 70-71 0.03971241110452363 72-73 0.043469850026326536 74-75 0.026507557393656404 76-77 0.0201815567089023 78-79 0.01955042439000572 80-81 0.0239487806278974 82-83 0.020215804199074986 84-85 0.012387806445318941 86-87 0.014374160875334536 88-89 0.018033749825215488 90-91 0.020504461616244737 92-93 0.019080744524780358 94-95 0.022720763480276917 96-97 0.02380689816861057 98-99 0.026835354799594938 100 0.02893423669732078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0219727E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.489084145232844 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82706039326584 47.383635574004416 2 14.601372622637857 18.248528049038324 3 4.852365234391669 9.096595783059106 4 2.091444141868685 5.227697158651462 5 0.9820128744962204 3.068254257304815 6 0.5507248973408649 2.0648576670484817 7 0.29750131079874925 1.3013409110674077 8 0.18856844735554965 0.9426775659149481 9 0.11623109874376787 0.653685741872296 >10 0.4402572427285617 4.568354665924858 >50 0.025284584348307205 1.1004013126387553 >100 0.023513109529439587 2.9457409812263835 >500 0.002536764000850395 1.0914113331073807 >1k 0.0010405647669675746 1.1037000703506894 >5k 2.1678431664568393E-5 0.08996332525755084 >10k+ 6.503529499370518E-5 1.1131556035331227 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36998 0.3620253261168327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27186 0.2660149336670148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17988 0.17601252949320467 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.784997192195056E-6 0.0 0.0 0.0 0.0 2 9.784997192195056E-6 0.0 0.0 0.0 0.0 3 9.784997192195056E-6 0.0 0.0 0.0 0.0 4 9.784997192195056E-6 0.0 0.0 0.0 0.0 5 9.784997192195056E-6 0.0 0.0 0.0 0.0 6 9.784997192195056E-6 0.0 0.0 0.0 0.0 7 9.784997192195056E-6 0.0 0.0 0.0 0.0 8 9.784997192195056E-6 0.0 0.0 9.784997192195056E-6 0.0 9 9.784997192195056E-6 0.0 0.0 9.784997192195056E-6 0.0 10-11 9.784997192195056E-6 0.0 0.0 9.784997192195056E-6 0.0 12-13 9.784997192195056E-6 0.0 0.0 2.9354991576585168E-5 0.0 14-15 9.784997192195056E-6 0.0 0.0 3.9139988768780224E-5 0.0 16-17 9.784997192195056E-6 0.0 0.0 4.892498596097528E-5 0.0 18-19 9.784997192195056E-6 0.0 0.0 5.381748455707281E-5 0.0 20-21 9.784997192195056E-6 0.0 0.0 1.076349691141456E-4 0.0 22-23 9.784997192195056E-6 0.0 0.0 3.229049073424368E-4 0.0 24-25 9.784997192195056E-6 0.0 0.0 6.898423020497515E-4 0.0 26-27 9.784997192195056E-6 0.0 0.0 0.001418824592868283 0.0 28-29 9.784997192195056E-6 0.0 0.0 0.004520668702794116 0.0 30-31 9.784997192195056E-6 0.0 0.0 0.017089497596168665 0.0 32-33 9.784997192195056E-6 0.0 0.0 0.04593077682016359 0.0 34-35 9.784997192195056E-6 0.0 0.0 0.08680760259055843 0.0 36-37 9.784997192195056E-6 0.0 0.0 0.1543876856984536 0.0 38-39 9.784997192195056E-6 0.0 0.0 0.2530889523761251 0.0 40-41 9.784997192195056E-6 0.0 0.0 0.3608266639607888 0.0 42-43 9.784997192195056E-6 0.0 0.0 0.4678598557476144 0.0 44-45 9.784997192195056E-6 0.0 0.0 0.5900744706781307 0.0 46-47 9.784997192195056E-6 0.0 0.0 0.7347603316605229 0.0 48-49 9.784997192195056E-6 0.0 0.0 0.89585074043563 0.0 50-51 9.784997192195056E-6 0.0 0.0 1.0661341540728047 0.0 52-53 9.784997192195056E-6 0.0 0.0 1.230654204363776 0.0 54-55 9.784997192195056E-6 0.0 0.0 1.408339968376846 0.0 56-57 1.9569994384390112E-5 9.784997192195056E-6 0.0 1.612817054702146 0.0 58-59 1.9569994384390112E-5 9.784997192195056E-6 0.0 1.850240226573567 0.0 60-61 1.9569994384390112E-5 9.784997192195056E-6 0.0 2.0931527818698092 0.0 62-63 1.9569994384390112E-5 9.784997192195056E-6 0.0 2.3374645917645354 0.0 64-65 1.9569994384390112E-5 9.784997192195056E-6 0.0 2.5870211601542783 0.0 66-67 1.9569994384390112E-5 9.784997192195056E-6 0.0 2.8630363609517167 0.0 68-69 1.9569994384390112E-5 9.784997192195056E-6 0.0 3.1661315414785545 0.0 70-71 1.9569994384390112E-5 9.784997192195056E-6 0.0 3.4946285747163306 0.0 72-73 1.9569994384390112E-5 9.784997192195056E-6 0.0 3.8272450917720207 0.0 74-75 2.446249298048764E-5 9.784997192195056E-6 0.0 4.145443415465012 0.0 76-77 2.9354991576585168E-5 9.784997192195056E-6 0.0 4.500350156124522 0.0 78-79 2.9354991576585168E-5 9.784997192195056E-6 0.0 4.882102036580821 4.892498596097528E-6 80-81 2.9354991576585168E-5 9.784997192195056E-6 0.0 5.295669835407541 9.784997192195056E-6 82-83 2.9354991576585168E-5 9.784997192195056E-6 0.0 5.712481360803473 9.784997192195056E-6 84-85 2.9354991576585168E-5 9.784997192195056E-6 0.0 6.1139206556104675 9.784997192195056E-6 86-87 2.9354991576585168E-5 9.784997192195056E-6 0.0 6.535228387216215 9.784997192195056E-6 88 2.9354991576585168E-5 9.784997192195056E-6 0.0 6.876142581890886 9.784997192195056E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19105 0.0 47.316967 1 GTATCAA 34730 0.0 40.92029 1 GTACTAG 10515 0.0 36.474125 1 GCATCGT 9610 0.0 35.85355 8 GGGCACC 11120 0.0 34.78273 94 CAACGCA 39615 0.0 34.079678 5 ATCAACG 39865 0.0 33.78358 3 TATCAAC 44430 0.0 31.78514 2 CTGTGCG 13280 0.0 31.749691 9 TAGGCAT 12870 0.0 31.55774 5 AACGCAG 45830 0.0 30.166311 6 TAGTACT 14195 0.0 30.10197 4 ACTGTGC 14905 0.0 29.613188 8 GTGGTAT 6140 0.0 29.124052 1 GTACATG 50530 0.0 29.047092 1 CTAGGCA 14555 0.0 28.485432 4 TCAACGC 48265 0.0 28.312561 4 TACATGG 51215 0.0 28.300102 2 ACATGGG 49975 0.0 27.776878 3 ACGCAGA 48605 0.0 27.76743 7 >>END_MODULE