FastQCFastQC Report
Mon 6 Jun 2016
ERR1211176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10219727
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329080.3220046876007549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262390.25674854132600605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175990.1722061655854408No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC111620.10922013865928121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199850.046.938431
GTATCAA355000.039.4444731
GTACTAG98550.034.877921
GGGCACC103100.033.694894
ATCAACG413350.032.3415683
CAACGCA413450.032.3316655
GTGGTAT62250.031.573791
TAGGCAT124550.030.2551575
TATCAAC462650.030.2123662
CTGTGCG131000.029.4825529
AACGCAG474300.029.1148976
TAGTACT141800.027.7790414
ACTGTGC147750.027.6984318
TCAACGC492450.027.5285024
CTAGGCA141000.027.4365924
GTACATG507900.026.3201561
ACGCAGA513000.026.1033527
TACATGG510000.026.0065772
CGCAGAG517300.025.831628
GCATCGT120450.025.745528
Produced by FastQC (version 0.11.5)