FastQCFastQC Report
Thu 26 May 2016
ERR489047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18070277
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1498880.8294726195951507No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA341230.1888349580916773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252720.13985397124792276No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC245990.13612962324816602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG285900.033.8019141
TACCTGG288600.033.035482
ACCTGGG324200.028.56053
CCTGGGG260250.021.7932284
GTACCCG35950.021.2476861
TACCCGG35650.020.2660662
GAGTACT550250.020.2268712
AGTACTT564300.019.68753413
TATAACG15050.018.8347592
GTACATG646900.018.8285561
AGAGTAC946200.018.77223811
GTACTTT596450.018.59263214
TACATGG659200.018.044722
GTACACG37700.017.9693761
ATAACGC17500.017.775043
TACTTTT646450.017.11526115
ACATGGG678350.016.9921423
TATCACG13950.016.8506772
ACTTTTT680200.016.7733316
TAACGCA19250.016.3385834
Produced by FastQC (version 0.11.5)