FastQCFastQC Report
Thu 26 May 2016
ERR489047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18070277
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1562460.8646574703863146No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA450210.2491439395201302No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA372960.20639417979038174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234320.12967150420549722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181440.10040797935748301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG288900.032.121921
TACCTGG291650.031.4902612
ACCTGGG326400.027.2495523
ATAACGC18950.022.5755883
CCTGGGG263650.021.0017074
TATAACG18100.020.7767732
TAACGCA20950.020.7488424
GTACCCG33950.019.3644031
TACCCGG32450.019.0313032
GAGTACT578900.018.92700612
AGTACTT595550.018.4206713
GTACTTT618200.017.64531514
AGAGTAC1006850.017.49185811
GTACATG652800.017.4648781
TACATGG663000.016.818532
TACTTTT667150.016.36076215
GTACACG36200.016.0579831
ACTTTTT692450.015.90746616
ACATGGG679700.015.8569763
GAGTACG33700.015.7091191
Produced by FastQC (version 0.11.5)