FastQCFastQC Report
Thu 26 May 2016
ERR489046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13836676
Sequences flagged as poor quality0
Sequence length75
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG208950.028.0664312
GTACCTG212800.028.0589491
GTATCAA544400.025.438721
GGTATCA361800.025.2975921
ACCTGGG237650.023.932113
TATAACG7800.022.1786582
TCAACGC671350.020.4499424
TATCAAC676350.020.3392432
ATCAACG678100.020.259243
CAACGCA683050.020.1103555
AACGCAG712350.019.3896796
GTACATG627200.018.8517041
GAGTACT325350.018.7886712
AGTACTT323200.018.75562313
GTACTTT322700.018.70947814
AGAGTAC698350.018.56779511
TACATGG638650.018.3652422
TACTTTT328250.018.3353415
ACTTTTT353300.017.36773716
GTATATA21750.017.2477171
Produced by FastQC (version 0.11.5)