FastQCFastQC Report
Thu 26 May 2016
ERR489046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13836676
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG207250.026.5573041
TACCTGG203950.026.4596122
GGTATCA368500.024.9907281
GTATCAA545400.024.3760661
ACCTGGG230750.022.9379233
TATAACG8100.020.446862
TCAACGC675700.019.562344
TATCAAC684950.019.3589552
ATCAACG683400.019.3523753
CAACGCA691050.019.1527755
GAGTACT344650.018.84869412
AGTACTT344150.018.70566213
GTACATG609050.018.6023031
AACGCAG717300.018.499976
GTACTTT349050.018.34423314
TACTTTT350600.018.21274815
TACATGG618800.018.1163142
AGAGTAC711350.017.85219611
ACTTTTT371150.017.26939816
CGTATAC5600.017.2520393
Produced by FastQC (version 0.11.5)