FastQCFastQC Report
Thu 26 May 2016
ERR489045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739226
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283780.13053822615395783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT750100.021.2913112
AGTACTT759200.020.8917313
GTACTTT767700.020.51515214
AGAGTAC1440650.019.89706611
GTATCAA1610050.019.8124221
GTACCTG254600.019.3852041
TACCTGG250700.019.325012
TACTTTT819450.019.22409615
ACTTTTT858000.018.8305916
GTACATG1149150.018.5881141
TACATGG1163550.018.1322612
TATAACG13900.017.9123942
ACATGGG1176700.017.37763
GGTATCA1168550.017.3116761
TATCAAC1863850.017.0376452
ATCAACG1874750.016.926963
TCAACGC1879100.016.8897614
CAACGCA1896700.016.6975785
AACGCAG1940300.016.378526
CATGGGT207950.016.159884
Produced by FastQC (version 0.11.5)