FastQCFastQC Report
Thu 26 May 2016
ERR489045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739226
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269210.1238360556166995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT769950.019.92023512
AGTACTT777000.019.68639613
GTACCTG246500.019.2460881
GTACTTT788000.019.2364714
TACCTGG242050.019.0281542
GTATCAA1601950.018.7244071
AGAGTAC1482150.018.50196511
TACTTTT838650.018.08673715
GTACATG1170150.017.7798141
ACTTTTT865800.017.70285216
TACATGG1179350.017.318072
GGTATCA1187200.017.2648681
TATCACG12300.017.109812
ACATGGG1188850.016.5993273
TATCAAC1887050.015.7997912
ATCAACG1891050.015.7280963
TCAACGC1896550.015.6897254
CAACGCA1915750.015.5236175
AACGCAG1960700.015.2057776
TATAACG14400.015.0937992
Produced by FastQC (version 0.11.5)