FastQCFastQC Report
Thu 26 May 2016
ERR489044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18194743
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA605700.3328983542114335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191240.10510728291133324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG207550.023.860281
TACCTGG206850.023.1964072
TATAACG9600.020.540142
GAGTACT522600.019.34906612
AGAGTAC892650.018.69619211
ACCTGGG246300.018.692873
AGTACTT540850.018.65283813
GTACATG658550.018.6097971
GTACTTT552200.018.20081314
TACATGG672350.017.8539312
ACATGGG683150.016.9979923
TACTTTT603550.016.67615
ACTTTTT626600.016.31700116
GTATCAA1199600.016.0264871
GTGTAGC89400.015.6547541
CATGGGG414850.014.8096774
ATAACGC16650.014.5427373
TATCAAC1355200.014.0167662
TCAACGC1357750.013.985154
ATCAACG1360000.013.9482873
Produced by FastQC (version 0.11.5)