FastQCFastQC Report
Thu 26 May 2016
ERR489044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18194743
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA639220.3513212580139219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191150.10505781807415471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG203500.023.7629641
TACCTGG208550.022.4172022
GAGTACT530750.019.55253812
AGTACTT547750.019.0401813
GTACATG647700.018.7452431
TATAACG11150.018.5664422
AGAGTAC908100.018.54724511
GTACTTT559500.018.44300314
ACCTGGG246050.018.3415783
TACATGG667050.017.8500272
TACTTTT605650.017.08191915
ACATGGG676050.016.959143
ACTTTTT624200.016.67375816
GTATCAA1236900.015.3335081
ATAACGC16300.014.393743
CATGGGG403650.014.1548514
GTGTAGC89400.014.045171
GTACCCG27700.013.8612081
TCAACGC1397000.013.45768454
TATCAAC1402000.013.449142
Produced by FastQC (version 0.11.5)