FastQCFastQC Report
Thu 26 May 2016
ERR489043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16959603
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2401551.4160414014408238No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA496570.2927957688632216No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG464870.27410429359696686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202980.1196844053484035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG279300.039.7480471
TACCTGG280900.038.9741672
ACCTGGG317000.033.7334983
CCTGGGG244900.026.8538384
GAGTACG43750.024.750461
TATAACG13550.023.4708632
TACGCAG44700.021.570144
TACACGG31250.020.7964082
ATAACGC15800.020.781023
GTACCCG34550.020.6270051
GTACACG35200.020.2461091
TACCCGG34250.020.0850542
GTACGCA51000.019.0430473
GTACATG494500.018.8682521
AGTACGC54150.018.7046552
GAGTACT454200.018.60830512
ACGCAGT51900.018.311955
TACATGG497700.018.3087332
TATCACG13850.018.2202192
AGTACTT474950.017.7521913
Produced by FastQC (version 0.11.5)