FastQCFastQC Report
Thu 26 May 2016
ERR489043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16959603
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2416751.4250038753855265No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC398230.23481092098677075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196070.1156100175222262No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG192410.11345194813817282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG288950.038.183291
TACCTGG293100.037.2620742
ACCTGGG325900.032.7079773
CCTGGGG253550.026.08164
TATAACG14300.025.0881822
ATAACGC16750.023.684413
GAGTACG43050.023.0503731
GTACCCG33700.021.54561
TAACGCA17000.021.3066964
TACGCAG42750.021.3031354
TACCCGG36000.019.9311682
ACGCAGT45900.019.765595
TACACGG30650.019.4709662
GTACACG34850.019.1480141
GTACGCA50100.018.5222663
TATCACG11950.018.1863082
TAGATCG12350.017.5970695
GAGTACT458700.017.53652412
TACCGGG18250.017.200872
CTGGGGG137750.017.1790545
Produced by FastQC (version 0.11.5)