FastQCFastQC Report
Thu 26 May 2016
ERR489042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11815144
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA445480.3770415324603746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212220.17961693907412385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156110.13212703967044329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG161200.028.6360761
TACCTGG158950.028.4372442
ACCTGGG176800.024.9165763
GAGTACT440800.020.11445612
AGTACTT448750.019.65992413
AGAGTAC707850.019.32941811
TATAACG9600.018.7615932
GTACATG460950.018.6078321
CCTGGGG146650.018.397924
GTACTTT481450.018.2453914
TACATGG466150.017.94442
GTACACG23800.017.1822241
GTACCCG17550.017.1797371
TACTTTT514800.017.0318915
ACATGGG476550.016.9619543
GTATCAA908650.016.9607431
TACACGG21400.016.6709772
ACTTTTT535550.016.56901216
TAACGCA12600.016.4927864
ATAACGC13550.015.8465933
Produced by FastQC (version 0.11.5)