FastQCFastQC Report
Thu 26 May 2016
ERR489042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11815144
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA467980.3960848890203962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209520.17733173628692125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169630.1435699810345096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124990.10578796161942673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG164300.027.6135671
TACCTGG163000.027.1583182
ACCTGGG184550.023.2765773
TATAACG10000.021.0471672
GAGTACT461500.019.44354612
AGTACTT471150.019.07459813
AGAGTAC758400.018.25937511
TAACGCA12300.017.9530684
GTACTTT498450.017.8845414
ATAACGC13650.017.694133
CCTGGGG143700.017.5519124
TACACGG21000.017.4160792
GTACATG477050.017.2737431
TACTTTT532750.016.7321615
TACATGG485150.016.5494732
GTACACG23850.016.5288541
GTATCAA955000.016.26171
ACTTTTT555750.016.04589816
ACATGGG493200.015.7896423
GTGTAGC68700.015.4528271
Produced by FastQC (version 0.11.5)