FastQCFastQC Report
Thu 26 May 2016
ERR489041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874274
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG210950.021.7138352
GTACCTG223900.020.9719811
GAGTACT519800.019.67538512
AGAGTAC941700.019.0622311
AGTACTT535550.018.96880713
GTACTTT541750.018.67606414
GTACATG774150.018.0761591
TACTTTT574300.017.59535615
TACATGG778700.017.5759242
GTATCAA1166500.017.5400811
GTATACG9550.017.353721
ACCTGGG259100.017.2106023
ACTTTTT619500.016.73196216
ACATGGG818300.016.1964423
GTATAGG45750.015.6973711
ACGTCGT13200.015.67846423
AACGTCG13300.015.55745122
TCAACGC1335500.015.1686694
TATCAAC1344800.015.1054582
ATCAACG1345150.015.0640693
Produced by FastQC (version 0.11.5)