FastQCFastQC Report
Thu 26 May 2016
ERR489041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20874274
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG210150.021.2306442
GTACCTG226850.020.3191341
GAGTACT539750.018.66973912
AGAGTAC966000.018.65986811
AGTACTT553850.018.126113
GTACTTT556550.017.88315414
GTACATG779000.017.4049661
GTATCAA1165250.017.318491
TACATGG784050.016.8909972
TACTTTT590050.016.86727515
AACGTCG13600.016.2337422
ACCTGGG265400.016.1859633
ACTTTTT628950.015.99408216
ACGTCGT13800.015.74849223
ACATGGG821250.015.6586433
GTATACG10950.015.4737571
TCAACGC1333650.014.8530554
TATCAAC1346750.014.8195612
ATCAACG1339000.014.8040173
CAACGCA1346350.014.6997125
Produced by FastQC (version 0.11.5)