FastQCFastQC Report
Thu 26 May 2016
ERR489040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234761
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263070.19877200653642327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207710.1569427660990629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA400600.034.5901341
GTATCAA701700.030.0352971
TATCAAC884300.023.716332
GTACCTG185950.023.3267151
TCAACGC898600.023.2937414
ATCAACG900500.023.2288783
TACCTGG185300.023.027992
CAACGCA910750.022.9332625
AACGCAG940950.022.3181236
GAGTACT526300.021.34287312
AGTACTT541600.020.86327713
GTACTTT555350.020.13377614
ACCTGGG208050.019.692793
ACGCAGA1075400.019.4703527
AGAGTAC1021200.019.40423211
CGCAGAG1092300.019.1412878
TACTTTT598500.018.74618715
GTACATG793600.018.4283121
ACTTTTT624750.018.24122616
CAGAGTA1122800.018.09051110
Produced by FastQC (version 0.11.5)