FastQCFastQC Report
Thu 26 May 2016
ERR489040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234761
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246970.18660707208841926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213640.1614233910230793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133110.10057605120334247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA419950.034.826861
GTATCAA703950.028.2000431
TACCTGG185550.022.5549142
GTACCTG192150.022.1897681
TATCAAC894950.022.1247712
ATCAACG904800.021.864853
TCAACGC906050.021.8454464
CAACGCA920400.021.4967865
AACGCAG953350.020.8877816
GAGTACT549950.019.75346212
AGTACTT558400.019.5355213
ACCTGGG213050.019.190143
GTACTTT566950.019.04010614
TATAACG9650.018.2340952
ACGCAGA1101300.018.0430777
AGAGTAC1059100.017.99647511
CGCAGAG1121900.017.7240078
TACTTTT610350.017.65796315
GTACATG797350.017.5093211
ACTTTTT629750.017.2838216
Produced by FastQC (version 0.11.5)