FastQCFastQC Report
Thu 26 May 2016
ERR489039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17341834
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG231400.026.7299522
GTACCTG243450.025.9098661
ACCTGGG257600.023.191993
GAGTACT517000.020.2713912
TATCACG12850.019.877062
AGTACTT535550.019.6151813
GTACATG777950.019.5226441
AGAGTAC965600.019.28885311
TACATGG787550.018.8895972
GTACTTT559400.018.61329714
ACATGGG812500.017.8619733
GTACACG37800.017.719291
TATAACG15350.017.3143392
CCTGGGG202300.017.2147144
TACTTTT612450.016.98614715
ACTTTTT633750.016.79925516
ATAACGC17000.016.6476043
TACACGG33400.016.6380712
CATGGGG468900.015.9949914
GAGTACG23800.015.3767761
Produced by FastQC (version 0.11.5)