FastQCFastQC Report
Thu 26 May 2016
ERR489039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17341834
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA974340.5618436896581988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG225200.026.9212472
GTACCTG239350.026.1524541
ACCTGGG257200.022.8191173
TATAACG13900.019.8594382
GAGTACT540450.019.7561812
ATAACGC16250.019.1097353
AGTACTT557850.019.08441513
AGAGTAC997150.018.9452711
GTACATG780700.018.7070031
GTACTTT580450.018.25809714
TACATGG794000.018.0916292
ACATGGG807350.017.2700063
CCTGGGG201400.016.8749394
TACTTTT632150.016.80780215
ACTTTTT649050.016.48177316
TATCACG11100.016.475732
TACACGG31500.015.774072
GTACACG38100.015.6124411
CATGGGG465950.015.3209844
TAACGCA19750.015.0243884
Produced by FastQC (version 0.11.5)