FastQCFastQC Report
Thu 26 May 2016
ERR489038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18039621
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2710051.5022765722184517No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA596850.3308550661901378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG246400.030.6092261
TACCTGG248800.029.7501142
ACCTGGG281000.025.6362973
GAGTACG45400.022.992671
TACGCAG43800.020.8346024
GAGTACT445900.019.5093712
TATAACG14100.019.3700312
AGTACTT458200.018.88899413
GTACATG737050.018.6882951
GTACTTT467050.018.46493514
CCTGGGG232300.018.4364594
ACGCAGT49450.018.3802365
TACATGG748700.018.1517092
AGAGTAC914850.017.99569311
GTACACG37600.017.282571
GTACCCG36100.017.04321
ACATGGG778700.017.0150223
TACTTTT504850.017.00686515
GTATAGG38800.016.7480561
ATAACGC17550.016.7419363
Produced by FastQC (version 0.11.5)