FastQCFastQC Report
Thu 26 May 2016
ERR489038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18039621
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2728081.5122712389578474No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA635670.35237436529292937No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC448140.24841985316653828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG240750.029.9060421
TACCTGG243300.029.1124482
ACCTGGG276450.024.8974363
GAGTACG42750.020.394711
CCTGGGG219100.018.7846684
TACGCAG41550.018.7647514
TATAACG13450.017.955922
GAGTACT457300.017.90196812
AGTACTT467650.017.51299513
GTACATG734650.017.0812721
ACGCAGT45700.016.9097065
GTACTTT486550.016.6341714
TACATGG747200.016.4840262
ATAACGC17200.016.4480233
AGAGTAC948900.016.29141211
GTATAGG40700.016.0664711
TACTTTT522550.015.57367415
ACATGGG776150.015.4600973
GTACACG36950.015.3561571
ACTTTTT543400.015.09674116
Produced by FastQC (version 0.11.5)