FastQCFastQC Report
Thu 26 May 2016
ERR489037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15502256
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA350050.225805844001028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314700.20300271134730324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231930.14961048250009548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157520.1016110171319581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG201000.025.3267671
TACCTGG201950.024.6996752
GTATCAA1039300.021.4738161
GAGTACT577200.020.95300112
ACCTGGG233700.020.4801793
AGTACTT594600.020.42790613
GGTATCA733150.020.219721
GTACTTT611150.019.71690414
AGAGTAC1058350.019.28892311
GTACATG765750.018.9099711
GTACCCG24900.018.6873321
TACTTTT653400.018.47764415
TATCAAC1220950.018.2187862
TACATGG787400.018.0827792
ACTTTTT679900.017.98305716
ATCAACG1235550.017.9423353
TCAACGC1240150.017.8777544
CAACGCA1257800.017.591225
AACGCAG1288700.017.2363726
ACATGGG802750.017.210963
Produced by FastQC (version 0.11.5)