FastQCFastQC Report
Thu 26 May 2016
ERR489037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15502256
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317450.20477664670226062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240400.15507420339336417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167950.10833907013276005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG196150.025.4855141
TACCTGG196950.024.9635052
GTATCAA1068250.020.9011381
GAGTACT601800.020.69892312
ACCTGGG233300.020.2901863
AGTACTT615300.020.1999913
GGTATCA759300.019.8785951
GTACTTT628850.019.68793714
AGAGTAC1085200.018.9297911
TACTTTT673500.018.2749215
GTACATG783450.018.2016431
ACTTTTT695650.017.75248116
TATCAAC1258350.017.6740282
TACATGG802350.017.6219832
ATCAACG1268950.017.4665723
TCAACGC1270950.017.427614
CAACGCA1289900.017.1957075
AACGCAG1321050.016.8241886
ACATGGG807550.016.7992843
GTACCCG26050.016.4675061
Produced by FastQC (version 0.11.5)