FastQCFastQC Report
Thu 26 May 2016
ERR489036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15272798
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213380.13971244823640042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG263850.034.850181
TACCTGG260200.034.635782
ACCTGGG292200.029.9991683
TATAACG14300.025.160652
CCTGGGG215650.024.5769564
ATAACGC17850.022.286533
TAACGCA17600.021.8187124
TACACGG32050.020.5092642
GAGTACT482750.020.19106312
TATCACG11350.019.8126262
AGTACTT497800.019.63066513
AGAGTAC829450.019.36233511
GTATCAA927150.019.1463051
GTACACG41550.019.1385331
GTACATG599250.019.0358161
TACATGG609600.018.4329812
GTACTTT529300.018.26719714
ACATGGG612150.017.6819653
GTACCCG29100.017.3029121
TACCCGG27950.017.2051122
Produced by FastQC (version 0.11.5)