FastQCFastQC Report
Thu 26 May 2016
ERR489036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15272798
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA408820.26767852229827177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207270.13571187152478545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG264900.034.9981881
TACCTGG264900.034.59552
ACCTGGG299000.029.980683
CCTGGGG222050.024.8305724
TATAACG12250.024.5051122
TATCACG12000.022.1402742
TACACGG30250.019.9611782
ATAACGC16250.019.7470873
GAGTACT495550.019.69501912
AGTACTT508800.019.18212713
GTACATG597950.018.7965261
AGAGTAC849250.018.5161111
TACCCGG26700.018.3506152
GTACTTT534150.018.16197214
TACATGG606900.018.0964342
GTATCAA958550.018.0013221
GTACCCG28100.017.7275581
ACATGGG611500.017.4411893
GTACACG41150.017.1495631
TAACGCA16900.016.9454124
Produced by FastQC (version 0.11.5)