FastQCFastQC Report
Thu 26 May 2016
ERR489035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685550
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8464745.763992496025004No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2583121.758953529149402No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1841241.2537766716261904No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1818061.2379924483591014No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1402920.9553064066378174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG67150.029.6146851
GCTTATG70500.029.4873811
AGTACGC68300.028.7951012
GTACGCA71900.027.3457163
TACGCAG73650.026.5534654
CGCAGTG80500.024.3660076
ACGCAGT81850.023.93395
GTGTAGC61600.023.6628531
TGCGACG4250.023.52610822
GTACCTG98600.022.66771
TAGCCCT93950.022.6548634
CTTATGT90550.022.5211522
TACCTGG99900.021.9356652
TATGTAC94300.021.801324
CGACGAG4600.021.73757624
TTATGTA94300.021.5462423
GTATAGC59850.020.3536131
ACCGTCG2402.910383E-1120.11343223
ACCTGGG109100.019.7319153
ATAGCCC52050.019.5178743
Produced by FastQC (version 0.11.5)