FastQCFastQC Report
Thu 26 May 2016
ERR489035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685550
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8445845.751122702248129No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1611561.0973780348710127No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG766350.5218394952861827No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT221040.15051530245717729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG68550.029.3686731
AGTACGC68000.029.023892
GCTTATG71450.027.9830061
GTACGCA71400.027.5934753
TACGCAG72400.027.163124
CGCAGTG77600.025.3873756
ACGCAGT79650.024.6473275
GTACCTG104000.024.2472781
TACCTGG102350.023.9690192
GTGTAGC64150.023.6181681
TAGCCCT93900.022.5603664
GTACCCG8400.022.2373621
CTTATGT89850.021.9273682
ACCTGGG113250.021.1441273
TATGTAC94350.020.7706364
TTATGTA95700.020.2264393
TGCGACG4450.019.37960822
ATAGCCC51800.018.650773
GTATAGC62100.018.6047421
GTAGCCC66850.018.5294063
Produced by FastQC (version 0.11.5)