FastQCFastQC Report
Thu 26 May 2016
ERR489034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13561593
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368310.27158313923740374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271480.20018297260506196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182040.134232018318202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG141100.024.6535991
GGTATCA595950.024.6307281
GTATCAA915150.024.4165331
TACCTGG141750.024.1714762
GAGTACT610050.021.29174212
AGTACTT616150.020.9982813
TATCAAC1080400.020.5896552
ACCTGGG163000.020.360083
ATCAACG1109400.020.0394693
TCAACGC1115250.019.9405274
GTACTTT650850.019.8029114
AGAGTAC1047000.019.70084211
CAACGCA1126500.019.695055
GTACATG733750.019.5243321
AACGCAG1155850.019.2828036
TACATGG743600.018.937722
ACATGGG746250.018.3399033
ACTTTTT737600.017.75914216
TACTTTT731200.017.63558215
ACGCAGA1280300.017.2979457
Produced by FastQC (version 0.11.5)