FastQCFastQC Report
Thu 26 May 2016
ERR489034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13561593
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351410.25912147636343313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285460.21049149609489093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195490.1441497322622792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA618300.025.0355781
TACCTGG144600.023.8350732
GTACCTG148950.023.8072241
GTATCAA927800.023.2830731
GAGTACT635200.020.34989212
ACCTGGG168600.020.0943163
AGTACTT644700.020.01278913
TATCAAC1100200.019.5579552
TATAACG11550.019.4155942
ATCAACG1120750.019.1685563
GTACTTT671200.019.13013614
TCAACGC1128650.019.0557844
CAACGCA1141450.018.8270535
AGAGTAC1089200.018.52850311
AACGCAG1167400.018.4288276
GTACATG724500.017.9864371
TACATGG734650.017.460162
ACTTTTT753100.017.15947516
TACTTTT751300.017.09497815
ACATGGG734350.016.7625873
Produced by FastQC (version 0.11.5)