FastQCFastQC Report
Thu 26 May 2016
ERR489033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10053178
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA415910.4137099731050221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260310.2589330458487853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178650.17770500034914333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127010.1263381589383974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG143800.024.8518332
GTACCTG150400.023.9661251
ACCTGGG149350.022.8855933
GAGTACT437250.021.25091212
AGTACTT447350.020.7873713
GTACATG634300.020.6009771
TACATGG643000.020.0415972
GTACTTT468450.019.74065814
CATGGGT112700.019.3403474
ACATGGG651000.019.30083
AGAGTAC797300.018.97080611
TACTTTT508150.018.2078515
GTATCAA925050.018.1801551
ACTTTTT516950.018.11731716
GTATAGG23250.017.922831
GGGTACC58450.017.516297
GGTACCT58800.017.4717948
TACCCGG12100.017.4406032
CATGGGG338100.017.437014
ATGGGTA73000.017.1487435
Produced by FastQC (version 0.11.5)