FastQCFastQC Report
Thu 26 May 2016
ERR489033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10053178
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255730.2543772725400863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188010.18701548903242338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133210.132505362980741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG145300.022.9867692
GTACCTG148850.022.8931961
ACCTGGG147100.021.4388623
GAGTACT447100.020.08534612
GTACACG19150.020.052721
AGTACTT456250.019.65229413
GTACATG643650.019.612961
TACATGG649850.019.2416922
GTACTTT473100.018.82109614
ACATGGG650950.018.7162253
TACCCGG11500.018.3020692
AGAGTAC821400.018.03924411
CATGGGT115200.017.7602394
TACTTTT511250.017.40202515
ACTTTTT517350.017.2701916
GTATCAA949900.017.1538681
TATCACG5250.017.0876462
TATAACG8900.017.058112
TACACGG20550.016.790222
GGGTACC59850.016.4272427
Produced by FastQC (version 0.11.5)