FastQCFastQC Report
Thu 26 May 2016
ERR489032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11211691
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA609510.5436378865596635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185620.1655593255290393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129040.11509414592321533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG173300.034.353082
GTACCTG177850.033.8879471
ACCTGGG194100.029.7242553
CCTGGGG141250.022.4389364
TATAACG10450.020.8427682
GAGTACT419100.020.17751512
GTACCCG15900.019.7900091
AGTACTT428500.019.75212913
ATAACGC13300.019.4934983
AGAGTAC674250.019.05932811
TACCCGG15400.018.8577422
GTACATG455850.018.6449681
TACATGG460100.018.109062
GTACTTT465150.018.07552514
TATCACG7700.017.9597552
TAACGCA13800.017.5323564
ACATGGG461700.017.3179263
GTACACG25800.017.155051
TACTTTT509150.016.50007215
ACTTTTT525450.016.37537216
Produced by FastQC (version 0.11.5)