FastQCFastQC Report
Thu 26 May 2016
ERR489032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11211691
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA623700.5562943181363097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180430.16093022899043508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135260.12064192636061769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG178050.033.4814032
GTACCTG182300.033.0937461
ACCTGGG195050.029.8557783
CCTGGGG140100.022.826724
GTACACG23900.020.866471
TACCCGG15200.020.426822
TACACGG23500.018.9375482
GAGTACT426400.018.17909412
TATAACG10150.018.0140652
GTACATG453300.017.793781
AGTACTT438500.017.6775413
GAGTACG16100.017.4238261
AGAGTAC688050.017.28262111
GTACCCG17800.017.121711
TACATGG460950.017.1015012
TATCACG6800.016.7419832
ACATGGG460950.016.4428883
GTACTTT468550.016.43336914
ATAACGC12600.015.0589283
GTGTAGC65400.014.9862561
Produced by FastQC (version 0.11.5)