FastQCFastQC Report
Thu 26 May 2016
ERR489031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25962376
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317210.12218065095428862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG316100.024.6449762
GTACCTG327800.024.3852041
GTACCCG38500.021.2923221
ACCTGGG363050.020.816873
TATAACG17750.020.4542062
GAGTACT896100.020.18976212
AGTACTT909500.019.85268613
GTACTTT941750.019.07246814
TACCCGG41750.018.6344992
GTACATG1168800.018.584671
AGAGTAC1570000.018.51796511
TACATGG1186450.018.0107062
GTGTAGC136500.017.6618231
TACTTTT1029150.017.48539215
TAGCCCT161500.017.3401074
ACTTTTT1081700.017.07398816
ACATGGG1193300.017.0559563
ATAACGC23150.017.0249393
GTATAGC98600.016.6278761
GTCCTAC96500.016.380391
Produced by FastQC (version 0.11.5)