FastQCFastQC Report
Thu 26 May 2016
ERR489031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25962376
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA987080.38019632717745094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299210.11524754128820874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG305000.023.5965272
GTACCTG313800.023.5250421
GTACCCG36950.020.6063331
ACCTGGG353950.019.4657253
GAGTACT917500.018.78114312
TACCCGG39000.018.6660082
AGTACTT932200.018.46704513
TATAACG16250.018.2590712
GTACTTT956350.017.85641914
GTACATG1174400.017.4254631
AGAGTAC1612500.017.26713411
TACATGG1186800.016.8755632
TACTTTT1033500.016.5401415
GTGTAGC141300.016.3126531
ACATGGG1187450.016.285263
TAGCCCT167900.016.1917614
ACTTTTT1086150.015.916244516
ATAACGC21000.015.7719853
GTCCTAC90550.015.4413541
GTAGGAC104600.015.4035073
Produced by FastQC (version 0.11.5)