FastQCFastQC Report
Thu 26 May 2016
ERR489030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11321312
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA821100.7252692974100529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247120.21827858820603124No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG182520.16121806377211403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178200.1574022516118273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120180.10615377440353203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT442600.020.9951812
TATAACG5800.020.8997062
AGTACTT448550.020.65628813
TACCTGG141550.020.3325232
GTACCTG145150.020.1244831
GTACTTT468400.019.65479914
GTACATG794000.019.0798031
GAGTACG14250.018.9667951
TACTTTT488500.018.82515515
TATCACG6450.018.7935352
AGAGTAC921600.018.69167911
TACATGG806900.018.5680772
ACTTTTT509750.018.22500416
ACATGGG816050.017.8286533
ACCTGGG148500.017.7010043
TACACGG21800.017.158032
CATGGGT135700.016.9727294
GGTACCT55550.015.9493798
CATGGGG435050.015.7708524
GTATAGG23300.015.7639191
Produced by FastQC (version 0.11.5)