FastQCFastQC Report
Thu 26 May 2016
ERR489030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11321312
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA863180.7624381343787716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242820.2144804418427829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190050.16786923635705825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129040.11397972249152749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT456200.020.90198112
AGTACTT460550.020.6596113
TATAACG6250.020.4249442
GTACCTG148600.020.2756021
TACCTGG149050.019.930142
GTACTTT477950.019.73425514
GTACATG803400.018.8459971
TACTTTT500350.018.82934615
GAGTACG13700.018.4321781
TACATGG817400.018.3777772
ACTTTTT517950.018.31606916
ACCTGGG149150.018.1356033
AGAGTAC957250.018.12068211
ACATGGG825100.017.5665023
CATGGGT138000.017.2760284
GGTACCT61150.016.2481028
TATCACG6250.016.008742
ATACCGT19700.015.762366
CATGGGG430800.015.5371374
ATGGGCG29200.015.4786615
Produced by FastQC (version 0.11.5)