FastQCFastQC Report
Thu 26 May 2016
ERR489029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21783996
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226480.10396623282523557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG322300.032.650361
TACCTGG325800.031.8745212
ACCTGGG379100.026.850033
GTATCAA861150.024.2028961
TATAACG15650.023.6354712
GGTATCA564350.022.8515931
CCTGGGG319800.020.965214
GAGTACT572700.020.0258612
TATCAAC1031400.019.9863072
TCAACGC1036200.019.8882794
ATCAACG1043250.019.7456343
CAACGCA1052900.019.567315
TAACGCA20400.019.4824544
ATAACGC22200.019.305543
AGTACTT595150.019.27085513
GTACCCG44250.019.1532231
AGAGTAC1004900.019.08407411
GTACATG761650.019.0621811
TACCCGG41050.019.0322482
AACGCAG1092500.019.010386
Produced by FastQC (version 0.11.5)