FastQCFastQC Report
Thu 26 May 2016
ERR489029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21783996
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218640.10036726044202358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG327550.033.3355371
TACCTGG331450.032.305432
ACCTGGG383650.027.1558593
GGTATCA588750.023.479281
GTATCAA878700.022.9915471
CCTGGGG314000.021.3465634
TATAACG13900.021.3430022
TATCAAC1056900.018.9502532
GAGTACT590000.018.83690312
TCAACGC1057400.018.8341964
ATCAACG1066300.018.6772943
CAACGCA1075250.018.5209375
GTACCCG44300.018.1854951
AGTACTT613900.018.15412313
AACGCAG1113600.017.9698526
AGAGTAC1046650.017.94450211
GTACATG769000.017.8679391
TACCCGG42650.017.7131962
GTACTTT624900.017.64136714
TACATGG779900.017.3299522
Produced by FastQC (version 0.11.5)