FastQCFastQC Report
Thu 26 May 2016
ERR489028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20732636
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383480.1849644203467422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268820.12966030947536047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451350.036.555131
GTATCAA810250.030.8582731
GTACCTG310050.030.2935641
TACCTGG304750.030.2670172
ACCTGGG355200.025.4295043
TATCAAC1025650.024.1380392
TCAACGC1040600.023.8916874
ATCAACG1039100.023.8657683
TATAACG15400.023.7964762
CAACGCA1058800.023.4779835
AACGCAG1098000.022.76786
GAGTACT723350.020.04090512
ACGCAGA1261000.019.763127
AGTACTT738700.019.5376413
CGCAGAG1282350.019.4083358
TATCACG14900.019.2583622
GTACATG875700.019.2004761
AGAGTAC1237950.019.10524211
CCTGGGG295450.018.9895844
TACATGG893750.018.4746282
Produced by FastQC (version 0.11.5)