FastQCFastQC Report
Thu 26 May 2016
ERR489028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20732636
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367910.17745452146075397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278580.13436786330498446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA455700.037.1180041
GTATCAA804550.030.2840371
GTACCTG307900.029.5132851
TACCTGG307550.029.0099932
ACCTGGG352350.024.6262633
TATCAAC1036950.023.3402062
TCAACGC1045650.023.0993164
ATCAACG1045950.023.07013
CAACGCA1070900.022.5444665
AACGCAG1110800.021.8745886
TATAACG14650.019.5467822
GAGTACT757450.019.29287712
CCTGGGG287100.018.950644
ACGCAGA1280250.018.9381337
AGTACTT769800.018.87630513
CGCAGAG1303700.018.5893278
AGAGTAC1296000.018.08221411
GTACATG876100.018.0349391
GTACTTT805600.017.92611914
GTGGTAT224950.017.5214561
Produced by FastQC (version 0.11.5)