FastQCFastQC Report
Thu 26 May 2016
ERR489027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954374
Sequences flagged as poor quality0
Sequence length75
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA580280.5829397207699851No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186500.18735482512511586No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG141150.14179696282257429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132490.1330972696022874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG140500.023.453392
GTACCTG148500.022.6750761
AGTACTT434600.021.45764213
GAGTACT439950.021.39911312
TATAACG6500.021.2782382
GTACTTT454650.020.4820614
TACTTTT459750.020.23699215
ACTTTTT486850.019.42216316
ACCTGGG172450.018.7642443
TAGATCG7600.018.6474885
AGAGTAC1003250.018.48887411
GATTTGA44400.018.10757661
GTGTAGC92650.017.9106771
GTAGCCC66550.017.6623843
GTACATG808000.017.2985061
CTCGATA5650.017.10810365
TACATGG812600.017.0162412
TATCACG4900.016.9357412
GTATATG13800.016.7846371
GTATAGG16700.016.7681351
Produced by FastQC (version 0.11.5)