FastQCFastQC Report
Thu 26 May 2016
ERR489027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954374
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA535910.5383663503099241No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171110.17189428486412106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137590.13822064551723695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG138100.023.708282
GTACCTG144350.023.0588511
AGTACTT440300.020.6218513
GAGTACT445000.020.4742312
TAGATCG6800.020.2942075
CTCGATA6050.019.9602765
GTACTTT458150.019.75816314
TACTTTT461000.019.598515
ACCTGGG169200.019.0859583
ACTTTTT483000.018.89865916
TATAACG6200.018.363262
CGATAAT6650.018.15934267
GATTTGA47300.017.79622561
AGAGTAC992050.017.5543611
ATACCGT19350.017.4732076
GTACATG769600.016.8280641
ACCGTCG20950.016.6324468
CTCGTAT35250.016.53925139
GTAGCCC67600.016.536243
TATGCCG37400.016.41862143
Produced by FastQC (version 0.11.5)