FastQCFastQC Report
Thu 26 May 2016
ERR489026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22930055
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374000.16310471126214043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258140.11257713947916828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG272800.026.0372261
TACCTGG269150.025.854892
ACCTGGG324700.021.0237923
GAGTACT760400.020.2399112
AGTACTT774100.019.86074313
TATAACG15900.019.785072
GTACATG948900.019.1125161
GTACTTT802750.019.01085714
GTACCCG39750.018.9559961
AGAGTAC1304850.018.88276911
TACATGG953150.018.7110252
TACCCGG39200.018.1666342
ACATGGG973200.017.7473493
TACTTTT865350.017.62691115
ACTTTTT919650.017.0137116
GTATAGG52500.016.064151
CCTGGGG269900.015.7901224
CATGGGG604500.015.7582584
GTGTAGC94450.014.235571
TAACGCA22350.013.76378254
Produced by FastQC (version 0.11.5)