FastQCFastQC Report
Thu 26 May 2016
ERR489026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22930055
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353260.15405981363760357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266750.11633203670902664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG271350.024.8763581
TACCTGG266350.024.8440462
TATAACG15250.020.3612
ACCTGGG321600.020.157393
GAGTACT772500.018.62247812
AGTACTT785850.018.2400713
GTACATG942500.017.8995321
AGAGTAC1326550.017.6893911
GTACTTT810000.017.62384414
TACATGG946300.017.4307612
ACATGGG958350.016.5382823
TACTTTT865900.016.46194315
TACCCGG40250.016.3717042
ACTTTTT904000.015.91696616
GTACCCG42650.015.8188511
TAACGCA19950.015.2176384
CCTGGGG264450.015.1981184
ATAACGC22500.015.0268213
CATGGGG594200.014.538154
TATACCG15950.014.4917435
Produced by FastQC (version 0.11.5)